Shi Wen-qi, Zhou Xiao-jun, Zhang Yi, Zhou Xiao-nong, Hu Ling, Wang Xue-zhong, Wang Jian, Li Yan-jun
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2011 Apr 30;29(2):134-7.
To reveal the distribution and composition of malaria-transmitting vectors on the western part of China-Myanmar border.
An entomological survey of malaria vectors was carried out in six villages of Yingjiang County and Xidong County on China-Myanmar border between August and September, 2008. Mosquitoes in human dwellings and cattle sheds were collected by overnight trapping with ovitrap light. The mosquitoes were firstly identified morphologically, and then Anopheles minimus A and C, An. aconitus, and An. jeyporiensis were identified by using multiplex PCR. Some mosquitoes were selected to extract the total genomic DNA, and detect sporozoites by nested PCR.
A total of 4571 mosquitoes were captured with 54.32% (2483/4571) of anopheline mosquitoes. There was significant difference in Anopheles species composition in human dwellings and cattle sheda The main species in human dwellings were An. kochi, An. minimus, and An. sinensis, while the principal species in cattle sheds consist of An. kochi (223), An. annularis (184), An. vagus (131), and An jeyporiensis (129). Furthermore, the composition in human dwellings of villages with and without cattle was significantly different. An. minimus (260) and An. kochi (49) werethe most important species in villages with cattle, whereas An. kochi (481) and An. sinensis (124) were the key species in villages without cattle. A total of 1075 mosquitoes were examined for sporozoites and 9 mosquitoes were found to be infected. Only three species, Le. An. minimus (7/408), An aconiaus (1/125) and An. pseudowillmori (1/101) were infected with malaria parasite. All sporozoites were identified as Plasmodium falcipoarum by sequencing, the target fragment was 204 bp.
The species composition of mosquitoes is complex in the study sites on the western part of China-Myanmar border, and An. minimus is the major malaria vector. Additionally, An. aconitus and An.pseudowillmori are also confirmed as potential malaria vector in this area.
揭示中国 - 缅甸边境西部疟疾传播媒介的分布及组成情况。
于2008年8月至9月在中国 - 缅甸边境的盈江县和西东县的6个村庄开展疟疾媒介昆虫学调查。采用诱蚊灯通宵诱捕法收集人房和牛舍内的蚊虫。先对蚊虫进行形态学鉴定，然后运用多重聚合酶链反应（PCR）鉴定微小按蚊A和C、中华按蚊、乌头按蚊及杰普尔按蚊。选取部分蚊虫提取全基因组DNA，采用巢式PCR检测子孢子。
共捕获4571只蚊虫，其中按蚊占54.32%（2483/4571）。人房和牛舍内按蚊种类组成存在显著差异。人房内的主要种类为高砂按蚊、微小按蚊和中华按蚊，而牛舍内的主要种类包括高砂按蚊（223只）、环纹按蚊（184只）、迷走按蚊（131只）和杰普尔按蚊（129只）。此外，有牛村庄与人房内的蚊虫种类组成与人房内无牛村庄的存在显著差异。微小按蚊（260只）和高砂按蚊（49只）是有牛村庄的最重要种类，而高砂按蚊（481只）和中华按蚊（124只）是无牛村庄的主要种类。共检测1075只蚊虫的子孢子，发现9只感染。仅微小按蚊（7/408）、乌头按蚊（1/125）和伪威氏按蚊（1/101）这三种按蚊感染疟原虫。经测序，所有子孢子均鉴定为恶性疟原虫，目标片段为204bp。
中国 - 缅甸边境西部研究地点蚊虫种类组成复杂，微小按蚊是主要的疟疾传播媒介。此外，乌头按蚊和伪威氏按蚊也被确认为该地区潜在的疟疾传播媒介。
