National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
Mol Plant. 2011 Nov;4(6):1105-22. doi: 10.1093/mp/ssr058. Epub 2011 Aug 10.
Plant phosphate transporter (PT) genes comprise a large family with important roles in various physiological and biochemical processes. In this study, a database search yielded 26 potential PT family genes in rice (Oryza sativa). Analysis of these genes led to identification of eight conserved motifs and 5-12 trans-membrane segments, most of them conserved. A total of 237 putative cis elements were found in the 2-kb upstream region of these genes. Of these, a majority were Pi-response and other stress-related cis regulatory elements, such as PHO-like, TATA-box-like, PHR1, or Helix-loop-helix elements, and WRKY1 and ABRE elements, suggesting gene regulation by these signals. Comprehensive expression analysis of these genes was performed using data from microarrays hybridized with RNA from 27 tissues covering the entire lifecycle from three rice genotypes: Minghui 63, Zhenshan 97, and Shanyou 63. Real-time PCR analysis confirmed that three rice PT genes are preferentially expressed in stamen at 1 d before flowering, two in panicle at the heading stage, and two in flag leaf at 14 d after the heading stage. Hormone-treatment experiments revealed differential up-regulation or down-regulation of 11 rice PT genes in seedlings exposed to five hormones, respectively. These results will be useful for elucidating the roles of these genes in the growth, development, and stress response of the rice plant.
植物磷酸盐转运蛋白（PT）基因家族庞大，在各种生理和生化过程中发挥着重要作用。本研究通过数据库搜索，在水稻（Oryza sativa）中鉴定出 26 个潜在的 PT 家族基因。对这些基因的分析确定了 8 个保守基序和 5-12 个跨膜片段，其中大多数是保守的。在这些基因的 2kb 上游区共发现 237 个假定顺式元件。其中，大多数是 Pi 反应和其他应激相关的顺式调控元件，如 PHO 样、TATA 盒样、PHR1 或螺旋-环-螺旋元件，以及 WRKY1 和 ABRE 元件，表明这些信号参与基因调控。使用来自三个水稻基因型（明恢 63、珍汕 97 和汕优 63）的 27 种组织的 RNA 杂交微阵列数据，对这些基因进行了综合表达分析。实时 PCR 分析证实，三个水稻 PT 基因在开花前 1d 的雄蕊中优先表达，两个在抽穗期的穗中表达，两个在抽穗后 14d 的旗叶中表达。激素处理实验表明，在幼苗中分别暴露于五种激素时，11 个水稻 PT 基因的表达水平出现差异上调或下调。这些结果将有助于阐明这些基因在水稻生长、发育和应激反应中的作用。
