CNRS UMR 8587, Laboratoire Analyse et Environnement, Université d'Évry Val d'Essonne, Évry, France.
Biochem Biophys Res Commun. 2011 Sep 9;412(4):561-4. doi: 10.1016/j.bbrc.2011.07.121. Epub 2011 Aug 4.
The detection of oligosaccharides at the single-molecule level was investigated using a protein nanopore device. Neutral oligosaccharides of various molecular weights were translocated through a single α-hemolysin nanopore and their nano-transit recorded at the single-molecule level. The translocation of maltose and dextran oligosaccharides featured by 1→4 and 1→6 glycosidic bonds respectively was studied in an attempt to discriminate oligosaccharides according to their polymerization degree and glycosidic linkages. Oligosaccharides were translocated through a free diffusion regime indicating that they adopted an extended conformation during their translocation in the nanopore. The dwell time increased with molecular mass, suggesting the usefulness of nanopore as a molecular sizing device.
使用蛋白质纳米孔设备研究了在单分子水平检测低聚糖。各种分子量的中性低聚糖通过单个α-溶血素纳米孔转运，并在单分子水平记录其纳米转运。研究了分别具有 1→4 和 1→6 糖苷键的麦芽糖和葡聚糖低聚糖的转运，试图根据其聚合度和糖苷键来区分低聚糖。低聚糖通过自由扩散模式转运，表明它们在纳米孔中转运时采用了伸展构象。停留时间随分子量的增加而增加，表明纳米孔作为分子尺寸测定装置的有用性。
