Biotechnology Center and Center for Regenerative Therapies Dresden, Dresden University of Technology, 01307 Dresden, Germany.
J Comp Neurol. 2012 Feb 15;520(3):633-55. doi: 10.1002/cne.22757.
The morphology of the telencephalon displays great diversity among different vertebrate lineages. Particularly the everted telencephalon of ray-finned fishes shows a noticeably different morphology from the evaginated telencephalon of nonray-finned fishes and other vertebrates. This makes the comparison between the different parts of the telencephalon of ray-finned fishes and other vertebrates difficult. Based on neuroanatomical, neurochemical, and connectional data no consensus on the subdivisions of the adult telencephalon of ray-finned fishes and their relation to nuclei in the telencephalon of other vertebrates has been reached yet. For tetrapods, comparative expression pattern analysis of homologous developmental genes has been a successful approach to clarify homologies between different parts of the telencephalon. In the larval zebrafish, subdivisions of the subpallium have been proposed using conserved developmental gene expression. In this study, we investigate the subdivisions of the adult zebrafish telencephalon by analyzing the expression pattern of conserved molecular marker genes. We identify the boundary between the pallium and subpallium based on the complementary expression of dlx2a, dlx5a in the subpallium and tbr1, neurod in the pallium. Furthermore, combinatorial expression of Isl, nkx2.1b, lhx1b, tbr1, eomesa, emx1, emx2, and emx3 identifies striatal-like, pallidal-like, and septal-like subdivisions within the subpallium. In contrast to previous models, we propose that the striatum and pallidum are stretched along the rostrocaudal axis of the telencephalon. Further, the septal nuclei derive from both the pallium and subpallium. On this basis, we present a new model for the subdivisions of the subpallium in teleost fish.
端脑的形态在不同的脊椎动物谱系中表现出很大的多样性。特别是硬骨鱼类的外翻端脑与非硬骨鱼类和其他脊椎动物的内陷端脑在形态上有明显的不同。这使得比较硬骨鱼类和其他脊椎动物的端脑的不同部分变得困难。基于神经解剖学、神经化学和连接数据，尚未就硬骨鱼类成年端脑的细分以及它们与其他脊椎动物端脑核的关系达成共识。对于四足动物，同源发育基因的比较表达模式分析已成为澄清端脑不同部分之间同源性的成功方法。在幼鱼斑马鱼中，使用保守的发育基因表达提出了亚皮层的细分。在这项研究中，我们通过分析保守分子标记基因的表达模式来研究成年斑马鱼端脑的细分。我们根据 dlx2a、dlx5a 在亚皮层中的互补表达以及 tbr1、neurod 在皮层中的表达，确定了皮层和皮层下之间的边界。此外，Isl、nkx2.1b、lhx1b、tbr1、eomesa、emx1、emx2 和 emx3 的组合表达在亚皮层内识别出纹状体样、苍白球样和隔室样细分。与以前的模型相比，我们提出纹状体和苍白球沿端脑的前后轴伸展。此外，隔核来源于皮层和皮层下。在此基础上，我们提出了硬骨鱼类亚皮层细分的新模型。
