用于一种世界性入侵杂草，孔雀草（菊科）的微卫星标记的分离和特征描述。
Isolation and characterization of microsatellite markers for a worldwide invasive weed, Chromolaena odorata (Asteraceae).
机构信息
Laboratory of Plant Phylogenetics and Conservation Biology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China.
出版信息
Am J Bot. 2011 Sep;98(9):e259-61. doi: 10.3732/ajb.1100169. Epub 2011 Aug 24.
PREMISE OF THE STUDY
Microsatellite loci were isolated and characterized for a worldwide invasive weed Chromolaena odorata (Asteraceae) to elucidate the population genetic structure and invasive history. •
METHODS AND RESULTS
A total of 14 microsatellite primer pairs were developed using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, and their polymorphism was assessed in two natural populations of C. odorata from Mexico and Trinidad and Tobago. Eleven loci showed polymorphism and eight of these loci were successfully amplified in Ageratina adenophora, another invasive weed related to C. odorata. •
CONCLUSIONS
These microsatellite markers are useful for investigating the population genetic structure and the history of range expansion of these invasive species.
研究前提
为了阐明全球入侵杂草臭菊（菊科）的种群遗传结构和入侵历史，我们分离并鉴定了其微卫星位点。
方法和结果
使用快速 AFLP 序列中重复片段分离（FIASCO）方案，总共开发了 14 对微卫星引物对，并在来自墨西哥和特立尼达和多巴哥的两个臭菊自然种群中评估了它们的多态性。11 个位点表现出多态性，其中 8 个位点在另一种与臭菊有关的入侵杂草银胶菊中成功扩增。
结论
这些微卫星标记可用于研究这些入侵物种的种群遗传结构和分布范围扩展的历史。
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