Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
Antiviral Res. 2011 Nov;92(2):237-46. doi: 10.1016/j.antiviral.2011.08.010. Epub 2011 Aug 16.
Carrageenan polysaccharide has been reported to be able to inhibit the infection and replication of many different kinds of viruses. Here, we demonstrated that a 2 kDa κ-carrageenan oligosaccharide (CO-1) derived from the carrageenan polysaccharide, effectively inhibited influenza A (H1N1) virus replication in MDCK cells (selectivity index >25.0). Moreover, the 2 kDa CO-1 inhibited influenza A virus (IAV) replication better than that of 3 kDa and 5 kDa κ-carrageenan oligosaccharides (CO-2 and CO-3). IAV multiplication was suppressed by carrageenan oligosaccharide treatment in a dose-dependent manner. Carrageenan oligosaccharide CO-1 did not bind to the cell surface of MDCK cells but inactivated virus particles after pretreatment. Different to the actions of carrageenan polysaccharide, CO-1 could enter into MDCK cells and did not interfere with IAV adsorption. CO-1 also inhibited IAV mRNA and protein expression after its internalization into cells. Moreover, carrageenan oligosaccharide CO-1 had an antiviral effect on IAV replication subsequent to viral internalization but prior to virus release in one replication cycle. Therefore, inhibition of IAV intracellular replication by carrageenan oligosaccharide might be an alternative approach for anti-influenza A virus therapy.
卡拉胶多糖已被报道能够抑制多种不同病毒的感染和复制。在这里，我们证明了一种来源于卡拉胶多糖的 2 kDa κ-卡拉胶低聚糖（CO-1），能够有效地抑制 MDCK 细胞中的甲型流感（H1N1）病毒复制（选择性指数>25.0）。此外，2 kDa CO-1 比 3 kDa 和 5 kDa κ-卡拉胶低聚糖（CO-2 和 CO-3）更能抑制甲型流感病毒（IAV）的复制。IAV 的增殖受到卡拉胶低聚糖处理的剂量依赖性抑制。卡拉胶低聚糖 CO-1 不与 MDCK 细胞表面结合，但在预处理后可使病毒颗粒失活。与卡拉胶多糖的作用不同，CO-1 可以进入 MDCK 细胞，并且不干扰 IAV 的吸附。CO-1 还在其内化到细胞后抑制 IAV mRNA 和蛋白表达。此外，卡拉胶低聚糖 CO-1 在一个复制周期中，在病毒内化后但在病毒释放之前，对 IAV 复制具有抗病毒作用。因此，卡拉胶低聚糖抑制 IAV 细胞内复制可能是抗甲型流感病毒治疗的一种替代方法。
