Crop Bioprotection, Research Units, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, USA.
J Microbiol. 2011 Aug;49(4):603-9. doi: 10.1007/s12275-011-1044-y. Epub 2011 Sep 2.
The objective of the study was to identify the lipopetides associated with three Bacillus subtilis strains. The strains are antagonists of Gibberella zeae, and have been shown to be effective in reducing Fusarium head blight in wheat. The lipopeptide profile of three B. subtilis strains (AS43.3, AS43.4, and OH131.1) was determined using mass spectroscopy. Strains AS43.3 and AS43.4 produced the anti-fungal lipopeptides from the iturin and fengycin family during the stationary growth phase. All three strains produced the lipopeptide surfactin at different growth times. Strain OH131.1 only produced surfactin under these conditions. The antifungal activity of the culture supernatant and individual lipopeptides was determined by the inhibition of G. zeae. Cell-free supernatant from strains AS43.3 and AS43.4 demonstrated strong antibiosis of G. zeae, while strain OH131.1 had no antibiosis activity. These results suggest a different mechanism of antagonism for strain OH131.1, relative to AS43.3 and AS43.4.
本研究旨在鉴定与三株枯草芽孢杆菌相关的脂肽。这些菌株是玉米赤霉的拮抗剂，已被证明能有效减少小麦赤霉病。采用质谱法测定了三株枯草芽孢杆菌（AS43.3、AS43.4 和 OH131.1）的脂肽图谱。在静止生长阶段，菌株 AS43.3 和 AS43.4 产生来自伊枯草菌素和芬枯草菌素家族的抗真菌脂肽。所有三株菌在不同的生长时间产生脂肽表面活性剂。在这些条件下，菌株 OH131.1 仅产生表面活性剂。通过抑制玉米赤霉来测定培养液上清液和单个脂肽的抗真菌活性。AS43.3 和 AS43.4 菌株的无细胞上清液对玉米赤霉具有很强的抑菌活性，而菌株 OH131.1 则没有抑菌活性。这些结果表明，与 AS43.3 和 AS43.4 相比，菌株 OH131.1 具有不同的拮抗机制。
