法属圭亚那的皮肤利什曼病：利用聚合酶链反应-限制性片段长度多态性技术修订流行病学情况
Cutaneous leishmaniasis in French Guiana: revising epidemiology with PCR-RFLP.
作者信息
Simon Stephane, Nacher Mathieu, Carme Bernard, Basurko Celia, Roger Amaury, Adenis Antoine, Ginouves Marine, Demar Magalie, Couppie Pierre
机构信息
Equipe EA3593 Ecosystemes Amazoniens et Pathologie Tropicale, Université de la Guyane, Campus Saint Denis, Avenue d'Estrées, 97300 Cayenne, French Guiana.
Laboratoire Associé - Centre National de Référence Leishmania, Laboratoire Hospito-Universitaire de Parasitologie Mycologie, Cayenne General Hospital, 97300 Cayenne, French Guiana.
出版信息
Trop Med Health. 2017 Feb 28;45:5. doi: 10.1186/s41182-017-0045-x. eCollection 2017.
BACKGROUND
The development of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique for species identification among patients presenting leishmaniasis allowed to better determine the main circulating species in French Guiana.
METHODS
A descriptive study of the species was identified, and their spatiotemporal distribution was conducted using patient records between 2006 and 2013, with 1017 new cases of leishmaniasis diagnosed. Identification was realized by PCR-RFLP on 745 cases.
RESULTS
The average proportions for different species were 86.2% for ; 9.7% for ; 2.8% for ; and 1.3% for , and no case of was identified. Over this period, the proportion of cases due to seemed to increase from 8.9% in 2006 to 13.0% in 2013 notably near the gold mining zones.
CONCLUSIONS
The use of molecular tools has transformed the view of the local epidemiology of cutaneous leishmaniasis in French Guiana.
背景
聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术用于利什曼病患者的物种鉴定，有助于更好地确定法属圭亚那主要的流行物种。
方法
对物种进行描述性研究，并利用2006年至2013年的患者记录对其时空分布进行研究，共诊断出1017例新的利什曼病病例。通过PCR-RFLP对745例病例进行鉴定。
结果
不同物种的平均比例分别为：[此处原文缺失该物种名称]占86.2%；[此处原文缺失该物种名称]占9.7%；[此处原文缺失该物种名称]占2.8%；[此处原文缺失该物种名称]占1.3%，未发现[此处原文缺失该物种名称]病例。在此期间，[此处原文缺失该物种名称]所致病例的比例似乎从2006年的8.9%增至2013年的13.0%，在金矿开采区附近尤为明显。
结论
分子工具的使用改变了法属圭亚那皮肤利什曼病当地流行病学的观点。
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