健康墨西哥孕妇的阴道微生物群由四种乳酸杆菌属物种和几种与阴道病相关的细菌组成。

Vaginal microbiota of healthy pregnant Mexican women is constituted by four Lactobacillus species and several vaginosis-associated bacteria.

作者信息

Hernández-Rodríguez César, Romero-González Roberto, Albani-Campanario Mario, Figueroa-Damián Ricardo, Meraz-Cruz Noemí, Hernández-Guerrero César

机构信息

Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Plan de Ayala s/n esq. Prol. Manuel Carpio, Colonia Santo Tomás, C.P. 11340 México, DF, Mexico.

出版信息

Infect Dis Obstet Gynecol. 2011;2011:851485. doi: 10.1155/2011/851485. Epub 2011 Sep 22.

OBJECTIVE

To identify the microbiota communities in the vaginal tracts of healthy Mexican women across the pregnancy.

METHODS

Vaginal swabs were obtained during the prenatal visit of women from all trimesters (n = 64) of healthy pregnant women of Mexico City. DNA was isolated from each sample, and PCR-DGGE and sequencing of 16S rRNA gene fragments were used to identify the bacterial communities.

RESULTS

21 different microorganisms were identified in the vaginal samples. Lactobacillus genus was present in 98% of women studied. Four lactobacilli species were identified in vaginal samples. L. acidophilus was the predominant (78%) followed by L. iners (54%), L. gasseri (20%), and L. delbrueckii (6%). 17 different microorganisms related to bacterial vaginosis conditions were identified. Ureaplasma urealyticum was the predominant (21%) followed by BVAB1 (17%) and Gemella bergeriae (7.8%).

CONCLUSIONS

Lactobacillus genus predominates in the vaginal samples of Mexican pregnant women associated with different microorganisms related to bacterial vaginosis conditions.

目的

确定墨西哥健康女性孕期阴道内的微生物群落。

方法

在墨西哥城健康孕妇所有孕期（n = 64）的产前检查时获取阴道拭子。从每个样本中分离DNA，并使用PCR-DGGE和16S rRNA基因片段测序来鉴定细菌群落。

结果

在阴道样本中鉴定出21种不同的微生物。在所研究的女性中，98%存在乳酸杆菌属。在阴道样本中鉴定出四种乳酸杆菌。嗜酸乳杆菌占主导（78%），其次是惰性乳杆菌（54%）、加氏乳杆菌（20%）和德氏乳杆菌（6%）。鉴定出17种与细菌性阴道病相关的不同微生物。解脲脲原体占主导（21%），其次是BVAB1（17%）和伯杰孪生球菌（7.8%）。