Takahashi K, Ghatei M A, Lam H C, O'Halloran D J, Bloom S R
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Diabetologia. 1990 May;33(5):306-10. doi: 10.1007/BF00403325.
Plasma concentrations of endothelin, a vasoconstrictor peptide released from vascular endothelial cells, have been measured by radioimmunoassay in 100 patients with diabetes mellitus and 19 healthy subjects. The plasma immunoreactive-endothelin concentrations were found to be greatly raised in the patients with diabetes (1,880 +/- 120 fmol/l, mean +/- SEM) compared with the healthy subjects (540 +/- 50 fmol/l, p less than 0.005). The elevation of immunoreactive-endothelin could not be explained by secondary changes in blood pressure or renal disease and did not correlate with the presence of diabetic retinopathy, duration of diabetes mellitus, fasting blood glucose or serum fructosamine. Fast protein liquid chromatographic analysis of the diabetic plasma immunoreactive-endothelin showed three forms, one in a very big molecular weight position, one intermediate and one in the position of endothelin-1 itself. No material appeared in the positions of endothelin-2 and 3. Chromatographic analysis of normal plasma showed only the big molecular weight peak while material in the endothelin-1, 2 or 3 positions was below detection. The elevation of endothelin in diabetic patients may be a marker of, and further exacerbate, their vascular disease.
血管内皮细胞释放的血管收缩肽内皮素的血浆浓度，已通过放射免疫分析法在100例糖尿病患者和19名健康受试者中进行了测定。结果发现，糖尿病患者的血浆免疫反应性内皮素浓度（1880±120 fmol/L，平均值±标准误）与健康受试者（540±50 fmol/L，p<0.005）相比大幅升高。免疫反应性内皮素的升高无法用血压或肾脏疾病的继发性变化来解释，且与糖尿病视网膜病变的存在、糖尿病病程、空腹血糖或血清果糖胺均无相关性。对糖尿病患者血浆免疫反应性内皮素进行快速蛋白质液相色谱分析显示有三种形式，一种处于非常大的分子量位置，一种处于中间位置，一种处于内皮素-1本身的位置。在内皮素-2和3的位置未出现物质。正常血浆的色谱分析仅显示出大分子量峰，而在内皮素-1、2或3位置的物质低于检测水平。糖尿病患者内皮素的升高可能是其血管疾病的一个标志物，并会进一步加重血管疾病。
