Department of Biological Sciences, Mississippi State University, Mississippi State, MS 39762, USA.
Plant Mol Biol. 2011 Nov;77(4-5):513-28. doi: 10.1007/s11103-011-9828-3. Epub 2011 Oct 11.
The soybean defense response to the soybean cyst nematode was used as a model to map at cellular resolution its genotype-defined cell fate decisions occurring during its resistant reactions. The defense responses occur at the site of infection, a nurse cell known as the syncytium. Two major genotype-defined defense responses exist, the G. max ([Peking])- and G. max ([PI 88788])-types. Resistance in G. max ([Peking]) is potent and rapid, accompanied by the formation of cell wall appositions (CWAs), structures known to perform important defense roles. In contrast, defense occurs by a potent but more prolonged reaction in G. max ([PI 88788]), lacking CWAs. Comparative transcriptomic analyses with confirmation by Illumina® deep sequencing were organized through a custom-developed application, Pathway Analysis and Integrated Coloring of Experiments (PAICE) that presents gene expression of these cytologically and developmentally distinct defense responses using the Kyoto Encyclopedia of Genes and Genomes (KEGG) framework. The analyses resulted in the generation of 1,643 PAICE pathways, allowing better understanding of gene activity across all chromosomes. Analyses of the rhg1 resistance locus, defined within a 67 kb region of DNA demonstrate expression of an amino acid transporter and an α soluble NSF attachment protein gene specifically in syncytia undergoing their defense responses.
以大豆抗大豆胞囊线虫的防御反应为模型，在细胞分辨率水平上对其在抵抗反应过程中发生的基因型定义的细胞命运决定进行作图。防御反应发生在感染部位，即称为合胞体的滋养细胞。存在两种主要的基因型定义的防御反应，G. max ([Peking])-和 G. max ([PI 88788])-型。G. max ([Peking])中的抗性强烈且迅速，伴随着细胞壁附属物（CWA）的形成，这些结构被认为具有重要的防御作用。相比之下，G. max ([PI 88788])中的防御反应则通过强烈但更持久的反应发生，缺乏 CWA。通过使用 Illumina®深度测序进行的比较转录组分析，并通过定制开发的应用程序，Pathway Analysis and Integrated Coloring of Experiments (PAICE) 进行了确认，该应用程序使用京都基因与基因组百科全书（KEGG）框架展示了这些细胞学和发育上不同的防御反应的基因表达。分析产生了 1643 个 PAICE 途径，从而更好地理解了所有染色体上的基因活性。对 rhg1 抗性基因座的分析，该基因座定义在 DNA 的 67 kb 区域内，表明在经历防御反应的合胞体中，特定表达氨基酸转运蛋白和α可溶性 NSF 附着蛋白基因。
