Department of Biological Chemistry, University of California Los Angeles, 615 Charles E Young Drive South, Los Angeles, CA90095, United States.
Department of Biological Chemistry, University of California Los Angeles, 615 Charles E Young Drive South, Los Angeles, CA90095, United States; Department of Physiology, University of California Los Angeles, 615 Charles E Young Drive South, Los Angeles, CA90095, United States; Howard Hughes Medical Institute, University of California Los Angeles, Los Angeles, CA90095, United States.
Curr Opin Struct Biol. 2020 Oct;64:51-58. doi: 10.1016/j.sbi.2020.05.018. Epub 2020 Jun 28.
Microcrystal electron diffraction (MicroED) was first coined and developed in 2013 at the Janelia Research Campus as a new modality in electron cryomicroscopy (cryoEM). Since then, MicroED has not only made important contributions in pushing the resolution limits of cryoEM protein structure characterization but also of peptides, small-organic and inorganic molecules, and natural-products that have resisted structure determination by other methods. This review showcases important recent developments in MicroED, highlighting the importance of the technique in fields of studies beyond protein structure determination where MicroED is beginning to have paradigm shifting roles.
微晶电子衍射（MicroED）于 2013 年在 Janelia 研究园区首次被提出并发展成为电子低温显微镜（cryoEM）的一种新模态。从那时起，MicroED 不仅在推动 cryoEM 蛋白质结构特征的分辨率极限方面做出了重要贡献，而且在其他方法难以确定结构的肽、小分子有机和无机分子以及天然产物方面也做出了重要贡献。本文展示了 MicroED 的重要最新进展，强调了该技术在除蛋白质结构测定以外的研究领域的重要性，在这些领域中，MicroED 开始发挥变革性的作用。
