Zheng Jiahao, Li Xiaohui, Zhou Shuhua, Zhang Qiangrong, Hu Kan, Liu Jinlai
Leqing City Hospital of Traditional Chinese Medicine, Wenzhou 325600, China.
Zhongguo Zhong Yao Za Zhi. 2011 Jun;36(12):1610-2.
To establish a high performance liquid chromatography method for the determination of tetrahydropulmatine content in treatment of winter's diseases in summer path.
The analysis was performed on the Hypersil ODS2 column (4.6 mm x 250 mm, 5 microm) with methanol and 0.1% phosphoric acid solution (adjusted pH to 6.28 with triethylamine) as mobile phase at 63:37. The flow rate was 1.0 mL x min(-1) and the UV detection wavelength was 280 nm.
The linear range of tetrahydropulmatine was obtained from the range of 2.08 - 49.92 mg x L(-1) (r = 0.999 8). The average recovery was 97.85% with RSD 2.49%.
This method is simple, reliable and can be used for the determination of tetrahydropulmatine in this preparation.
建立一种高效液相色谱法测定冬病夏治制剂中延胡索乙素的含量。
采用Hypersil ODS2柱（4.6 mm×250 mm，5μm），以甲醇和0.1%磷酸溶液（用三乙胺调pH至6.28）为流动相，比例为63:37。流速为1.0 mL·min⁻¹，紫外检测波长为280 nm。
延胡索乙素的线性范围为2.08～49.92 mg·L⁻¹（r = 0.999 8）。平均回收率为97.85%，相对标准偏差为2.49%。
该方法简便、可靠，可用于该制剂中延胡索乙素的含量测定。
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