[高效液相色谱法测定冬病夏治穴位敷贴中延胡索乙素的含量]
[Determination of tetrahydropulmatine content in treatment of winter's diseases in summer path by HPLC].
作者信息
Zheng Jiahao, Li Xiaohui, Zhou Shuhua, Zhang Qiangrong, Hu Kan, Liu Jinlai
机构信息
Leqing City Hospital of Traditional Chinese Medicine, Wenzhou 325600, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2011 Jun;36(12):1610-2.
OBJECTIVE
To establish a high performance liquid chromatography method for the determination of tetrahydropulmatine content in treatment of winter's diseases in summer path.
METHOD
The analysis was performed on the Hypersil ODS2 column (4.6 mm x 250 mm, 5 microm) with methanol and 0.1% phosphoric acid solution (adjusted pH to 6.28 with triethylamine) as mobile phase at 63:37. The flow rate was 1.0 mL x min(-1) and the UV detection wavelength was 280 nm.
RESULT
The linear range of tetrahydropulmatine was obtained from the range of 2.08 - 49.92 mg x L(-1) (r = 0.999 8). The average recovery was 97.85% with RSD 2.49%.
CONCLUSION
This method is simple, reliable and can be used for the determination of tetrahydropulmatine in this preparation.
目的
建立一种高效液相色谱法测定冬病夏治制剂中延胡索乙素的含量。
方法
采用Hypersil ODS2柱（4.6 mm×250 mm，5μm），以甲醇和0.1%磷酸溶液（用三乙胺调pH至6.28）为流动相，比例为63:37。流速为1.0 mL·min⁻¹，紫外检测波长为280 nm。
结果
延胡索乙素的线性范围为2.08～49.92 mg·L⁻¹（r = 0.999 8）。平均回收率为97.85%，相对标准偏差为2.49%。
结论
该方法简便、可靠，可用于该制剂中延胡索乙素的含量测定。
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