Institut Pasteur, Unité de Prévention et Thérapies Moléculaires des Maladies Humaines, CNRS-URA 3012, 25 rue du Dr Roux 75724 Paris, cedex 15, France.
J Clin Microbiol. 2011 Dec;49(12):4347-8. doi: 10.1128/JCM.01272-11. Epub 2011 Oct 19.
Pertussis is routinely diagnosed with real-time PCR based on insertion sequence IS481, which is not specific for Bordetella pertussis. We conducted a retrospective study using real-time PCRs specific for Bordetella pertussis and for Bordetella holmesii on 177 samples positive for IS481 PCR. Bordetella holmesii DNA was detected in 20.3% samples collected from adolescents and adults.
百日咳通常通过基于插入序列 IS481 的实时 PCR 进行诊断,但这种方法并不特异针对百日咳博德特氏菌。我们对 177 份 IS481 PCR 阳性样本进行了回顾性研究,使用针对百日咳博德特氏菌和博氏霍尔德菌的实时 PCR 方法。在从青少年和成年人中采集的样本中,有 20.3% 检测到博氏霍尔德菌 DNA。