Department of Applied Cosmetology and Graduate Institute of Cosmetic Science, Hungkuang University, Taiwan.
Electrophoresis. 2011 Nov;32(22):3157-63. doi: 10.1002/elps.201100343. Epub 2011 Oct 20.
A temperature-controlled microfluidic approach was developed for fabricating monodispersed agar beads with the potential to be a brand-new strategy for cultivating Cordyceps militaris. The proposed microfluidic system features a circulating water bath with precise temperature control (temperature deviation ▵T<0.1°C). This device holds the promise of allowing us to develop a temperature-controlled system, characterized as simple, low cost, and easy to set up and use. The size-controllable agar beads were achieved by utilizing microfluidic emulsification in the cross-junction channel under temperature-controlled conditions. The flow conditions of the dispersed/continuous phases were adjusted to generate various sizes of agar beads. Our results show that the microparticles produced are as small as 176 μm with a 95% particle size distribution within 5 μm. The prepared agar microparticles performed well as a substrate for the cultivation of C. militaris. The proposed method could also be applied for encapsulating biomaterials, enzymes, drugs, catalysts, and nanoparticles into agar beads for biomedical applications.
一种控温微流控方法被开发用于制备单分散琼脂珠，这可能成为一种新的蛹虫草培养策略。所提出的微流控系统具有精确控温的循环水浴（温度偏差 ▵T<0.1°C）。该设备有望使我们能够开发出一种控温系统，其特点是简单、低成本、易于设置和使用。在控温条件下，通过在十字形通道中进行微流乳化，可以实现尺寸可控的琼脂珠。调整分散/连续相的流动条件以产生不同大小的琼脂珠。我们的结果表明，所制备的微球小至 176μm，粒径分布在 5μm 内的占 95%。所制备的琼脂微球作为蛹虫草培养的基底表现良好。该方法还可用于将生物材料、酶、药物、催化剂和纳米颗粒包封到琼脂珠中，用于生物医学应用。
