Pharmaco-Biological Department, University of Messina, Italy.
Nat Prod Res. 2012;26(22):2132-6. doi: 10.1080/14786419.2011.628669. Epub 2011 Oct 21.
This study reported the antimicrobial activity and phenolic content of natural site and micropropagated Limonium avei (De Not.) Brullo & Erben inflorescences. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ethanolic extracts were determined according to the Clinical Laboratory Standards Institute guidelines. Individual phenolic acids and flavonoids were detected by a high performance liquid chromatography (HPLC-DAD) method. The samples showed a comparable antimicrobial activity, although the natural site extract possessed the lower MIC values. The best activity was detected against Gram-positive bacteria, such as Staphylococcus aureus including methicillin resistant strains (MIC and MBC values ranging from 7.81 to 62.50 µg mL(-1) and from 500 to 2000 µg mL(-1) respectively). In contrast, a low activity was found on Gram-negative bacteria and Candida albicans. The HPLC-DAD analysis revealed ten phenolic acids and four flavonoids with a major amount of m-coumaric acid, naringin and quercetin in the natural site extract.
本研究报告了天然生境和微繁殖的 Limonium avei（De Not.）Brullo & Erben 花序的抗菌活性和酚类含量。根据临床实验室标准协会指南，确定了乙醇提取物的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。通过高效液相色谱（HPLC-DAD）法检测了个别酚酸和类黄酮。尽管天然生境提取物的 MIC 值较低，但样品显示出相当的抗菌活性。对革兰氏阳性菌（如金黄色葡萄球菌，包括耐甲氧西林菌株）的活性最佳，MIC 和 MBC 值范围分别为 7.81 至 62.50μgmL-1和 500 至 2000μgmL-1。相比之下，对革兰氏阴性菌和白色念珠菌的活性较低。HPLC-DAD 分析显示，天然生境提取物中含有十种酚酸和四种类黄酮，其中主要的成分是 m-对香豆酸、柚皮苷和槲皮素。
