Chiang Mai University, Chiang Mai 50200, Thailand.
Pharm Biol. 2011 Nov;49(11):1190-203. doi: 10.3109/13880209.2011.576347.
The galls of Terminalia chebula Retz. (Combretaceae) frequently appear in many Thai Lanna medicinal plant recipes for promotion of longevity.
The objective of this study was to evaluate the skin anti-aging of gel containing niosomes loaded with a semi-purified fraction containing gallic acid from T. chebula galls.
The semi-purified fraction containing phenolic compounds including gallic acid isolated from T. chebula galls loaded in non-elastic or elastic niosomes, and its developed gel, were evaluated for rabbit skin irritation by the closed patch test and skin anti-aging in human volunteers by measuring skin elasticity and roughness.
Gel containing the fraction unloaded (SS) or loaded in non-elastic (SN) or elastic (SE) niosomes and gallic acid loaded in non-elastic (GN) or elastic (GE) niosomes showed no skin irritation, whereas the unloaded gallic acid (GS) gave the irritation in rabbit's skin by the closed patch test. The % parameter changes of skin elastic recovery and skin elastic extension when applied with SN and SE gels were +28.73 and +32.57; -21.25 and -22.63%, respectively. SN and SE gel also showed a significant decrease of the maximum and average roughness values with the parameter changes of -29.43 and -32.38; -39.47 and -35.28%, respectively.
The semi-purified fraction loaded in niosomes indicated not only higher chemical stability of gallic acid containing in the fraction, but also more in vivo anti-aging activities than the unloaded fraction when incorporated in gel.
诃子（使君子科）的虫瘿常出现在许多泰北兰纳传统药物配方中，用于延年益寿。
本研究旨在评估含有半纯化部位的尼奥斯omes 凝胶对皮肤的抗衰老作用，该半纯化部位含有来自诃子虫瘿的没食子酸。
从诃子虫瘿中分离出含有酚类化合物的半纯化部位，包括没食子酸，将其载入非弹性或弹性尼奥斯omes 中，并制备其凝胶，通过封闭贴斑试验评估对兔皮肤的刺激性，通过测量皮肤弹性和粗糙度评估对人体志愿者的皮肤抗衰老作用。
未载入（SS）或载入非弹性（SN）或弹性（SE）尼奥斯omes 的部位、以及载入非弹性（GN）或弹性（GE）尼奥斯omes 的没食子酸的凝胶均未引起皮肤刺激，而未载入的没食子酸（GS）则引起了兔皮肤的刺激。当使用 SN 和 SE 凝胶时，皮肤弹性恢复和皮肤弹性伸展的%参数变化分别为+28.73 和+32.57；-21.25 和-22.63%。SN 和 SE 凝胶还显著降低了最大和平均粗糙度值，参数变化分别为-29.43 和-32.38；-39.47 和-35.28%。
载入尼奥斯omes 的半纯化部位不仅表明含有该部位的没食子酸具有更高的化学稳定性，而且当载入凝胶中时，比未载入的部位具有更强的体内抗衰老活性。
