[Quantitative determination of contents of three components in Brucea javanica by HPLC].
作者信息
Zhou Zhongliu, Shi Renbing, Liu Bin, Zou Jieming, Wang Lisheng, Xia Jingmin
机构信息
Chemistry Science and Technology School, Zhanjiang Normal University, Zhanjiang 524048, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(14):1979-81.
OBJECTIVE
To develop an HPLC method for quantitative determination of three quassinoids in Brucea javanic.
METHOD
The determination was carried out on a phenomenex C18 column (4.6 mm x 250 mm, 5 microm) with gradient elution program of methol-water at a flow rate of 1.0 mL x min(-1), and the detection wavelength was 270 nm.
RESULT
Linearites of bruceine D, brusatol and bruceine H were good (r = 0.9996, 0.9996, and 0.9998) in ranges of 2.52-12.60, 2.19-10.95, and 2.91-14.55 microg, respectively. The average recoveries of bruceine D, brusatol and bruceine H were 100.01%, 100.95% and 100.43% respectively, and RSD of the above three compounds were 0.31% (n = 6), 1.7% ( n = 6) and 1.7% (n = 6), respectively.
CONCLUSION
The determination results of three batch samples showed that the method was simple, accurate and could be used in the quantitative determination of three components in the B. javanica.
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