Takashima T, Ueta C, Tsuyuguchi I, Kishimoto S
Osaka Prefectural Habikino Hospital, Japan.
Infect Immun. 1990 Oct;58(10):3286-92. doi: 10.1128/iai.58.10.3286-3292.1990.
We studied the production of tumor necrosis factor alpha (TNF-alpha) by peripheral blood monocytes taken from patients with pulmonary tuberculosis and from healthy controls. It was found that the monocytes from patients with newly diagnosed tuberculosis released significantly greater amounts of TNF-alpha in vitro in response to lipopolysaccharide than did those from healthy controls (P less than 0.05). However, the monocytes from patients with chronic refractory tuberculosis released significantly lower amounts of TNF-alpha than did those from patients with newly diagnosed tuberculosis (P less than 0.005). Even when the cells were primed for 24 h with 500 U of recombinant interferon gamma per ml, the same pattern of results was observed. The depressed TNF-alpha production by the monocytes from patients with chronic refractory tuberculosis was also shown in response to Mycobacterium bovis BCG. This depressed TNF-alpha production did not recover, even when cultured for 1 to 7 days in the sera of healthy individuals. The sera from patients with chronic refractory tuberculosis did not have any suppressive effect on the lipopolysaccharide-induced TNF-alpha production. Thus, it was demonstrated that the levels of TNF-alpha produced by monocytes were related to the disease states of pulmonary tuberculosis and that the depressed TNF-alpha production by monocytes in patients with chronic refractory tuberculosis might not be acquired.
我们研究了从肺结核患者和健康对照者采集的外周血单核细胞中肿瘤坏死因子α（TNF-α）的产生情况。结果发现，新诊断肺结核患者的单核细胞在体外对脂多糖刺激释放的TNF-α量显著多于健康对照者（P<0.05）。然而，慢性难治性肺结核患者的单核细胞释放的TNF-α量显著低于新诊断肺结核患者（P<0.005）。即使细胞用每毫升500单位的重组干扰素γ预处理24小时，也观察到相同的结果模式。慢性难治性肺结核患者的单核细胞对牛分枝杆菌卡介苗的反应也显示出TNF-α产生受抑制。即使在健康个体的血清中培养1至7天，这种TNF-α产生受抑制的情况也没有恢复。慢性难治性肺结核患者的血清对脂多糖诱导的TNF-α产生没有任何抑制作用。因此，证明单核细胞产生的TNF-α水平与肺结核的疾病状态有关，并且慢性难治性肺结核患者单核细胞TNF-α产生受抑制可能不是后天获得的。
