Suzuki Nao, Yoneda Masahiro, Hatano Yuko, Iwamoto Tomoyuki, Masuo Yosuke, Hirofuji Takao
Section of General Dentistry, Department of General Dentistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 801-0193, Japan.
Int J Dent. 2011;2011:834151. doi: 10.1155/2011/834151. Epub 2011 Oct 26.
This study investigated the inhibitory effect of probiotic Enterococcus faecium WB2000 on biofilm formation by cariogenic streptococci. The ability of E. faecium WB2000 and JCM5804 and Enterococcus faecalis JCM5803 to inhibit biofilm formation by seven laboratory oral streptococcal strains and 13 clinical mutans streptococcal strains was assayed. The Enterococcal strains inhibited biofilm formation in dual cultures with the mutans streptococcal strains Streptococcus mutans Xc and Streptococcus sobrinus JCM5176 (P < 0.05), but not with the noncariogenic streptococcal strains. Enterococcus faecium WB2000 inhibited biofilm formation by 90.0% (9/10) of the clinical S. mutans strains and 100% (3/3) of the clinical S. sobrinus strains. After culturing, the pH did not differ between single and dual cultures. The viable counts of floating mutans streptococci were lower in dual cultures with E. faecium WB2000 than in single cultures. Enterococcus faecium WB2000 acted as a probiotic bacterial inhibitor of cariogenic streptococcal biofilm formation.
本研究调查了益生菌屎肠球菌WB2000对致龋链球菌生物膜形成的抑制作用。测定了屎肠球菌WB2000、JCM5804以及粪肠球菌JCM5803对7株实验室口腔链球菌菌株和13株临床变形链球菌菌株生物膜形成的抑制能力。这些肠球菌菌株在与变形链球菌菌株变形链球菌Xc和远缘链球菌JCM5176的共培养中抑制了生物膜形成（P<0.05），但在与非致龋链球菌菌株共培养时则没有。屎肠球菌WB2000抑制了90.0%（9/10）的临床变形链球菌菌株和100%（3/3）的临床远缘链球菌菌株的生物膜形成。培养后，单培养和共培养之间的pH没有差异。与屎肠球菌WB2000共培养时，浮游变形链球菌的活菌数低于单培养。屎肠球菌WB2000作为一种益生菌，可抑制致龋链球菌生物膜的形成。
