包含五个钴胺素基因的反硝化假单胞菌5.4千碱基DNA片段的核苷酸序列以及编码S-腺苷-L-甲硫氨酸：尿卟啉原III甲基转移酶和钴胺酸a，c-二酰胺合酶的结构基因的鉴定。
Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase.
作者信息
Crouzet J, Cauchois L, Blanche F, Debussche L, Thibaut D, Rouyez M C, Rigault S, Mayaux J F, Cameron B
机构信息
Institut des Biotechnologies, Rhône-Poulenc Santé, Centre de Recherche de Vitry, France.
出版信息
J Bacteriol. 1990 Oct;172(10):5968-79. doi: 10.1128/jb.172.10.5968-5979.1990.
Abstract
A 5.4-kilobase DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment carries five different cob genes (cobA to cobE). Four of these genes present the characteristics of translationally coupled genes. cobA has been identified as the structural gene of S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase (SUMT) because the encoded protein has the same NH2 terminus and molecular weight as those determined for the purified SUMT. For the same reasons the cobB gene was shown to be the structural gene for cobyrinic acid a,c-diamide synthase. Genetic and biochemical data concerning cobC and cobD mutants suggest that the products of these genes are involved in the conversion of cobyric acid to cobinamide.
摘要
一个携带反硝化假单胞菌cob基因的5.4千碱基DNA片段已被测序。核苷酸序列和遗传分析表明，该片段携带五个不同的cob基因（cobA至cobE）。其中四个基因具有翻译偶联基因的特征。cobA已被鉴定为S-腺苷-L-甲硫氨酸：尿卟啉原III甲基转移酶（SUMT）的结构基因，因为编码的蛋白质具有与纯化的SUMT相同的NH2末端和分子量。出于同样的原因，cobB基因被证明是钴胺酸a，c-二酰胺合酶的结构基因。关于cobC和cobD突变体的遗传和生化数据表明，这些基因的产物参与了钴胺酸向辅酶维生素B12的转化。
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