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一种用于在临床样本中测定和定量各巨细胞病毒糖蛋白 H 亚型的新型实时 PCR 方法。

A novel real-time PCR method for determination and quantification of each cytomegalovirus glycoprotein H subtype in clinical samples.

机构信息

Department of Microbiology, Fukushima Medical University, School of Medicine, Fukushima, Japan.

出版信息

J Clin Microbiol. 2012 Feb;50(2):499-501. doi: 10.1128/JCM.06032-11. Epub 2011 Nov 23.

DOI:10.1128/JCM.06032-11
PMID:22116147
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3264147/
Abstract

To investigate reinfection in patients with congenital cytomegalovirus (CMV) infection, we established a CMV subtype-specific real-time quantitative PCR method targeting the CMV gH epitope region that can be used for evaluating pathogenic CMV strains in cases of mixed CMV infection.

摘要

为了调查先天性巨细胞病毒（CMV）感染患者的再感染情况，我们建立了一种针对 CMV gH 表位区域的 CMV 亚型特异性实时定量 PCR 方法，可用于评估混合 CMV 感染病例中的致病性 CMV 株。

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引用本文的文献

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J Virol. 2021 Aug 25;95(18):e0065721. doi: 10.1128/JVI.00657-21.

本文引用的文献

1
Screening for congenital cytomegalovirus infection using newborn urine samples collected on filter paper: feasibility and outcomes from a multicentre study.应用滤纸采集新生儿尿液样本筛查先天性巨细胞病毒感染：一项多中心研究的可行性和结果。
BMJ Open. 2011 Jul 29;1(1):e000118. doi: 10.1136/bmjopen-2011-000118.
2
Single cytomegalovirus strain associated with fetal loss and then congenital infection of a subsequent child born to the same mother.同一位母亲所生的后续孩子发生胎儿丢失和随后先天性感染，与单一巨细胞病毒株相关。
J Clin Virol. 2010 Oct;49(2):134-6. doi: 10.1016/j.jcv.2010.06.021. Epub 2010 Aug 3.
3
Cytomegalovirus reinfections in healthy seroimmune women.健康血清免疫女性的巨细胞病毒再感染。
J Infect Dis. 2010 Feb 1;201(3):386-9. doi: 10.1086/649903.
4
Birth prevalence and natural history of congenital cytomegalovirus infection in a highly seroimmune population.高血清免疫人群中先天性巨细胞病毒感染的出生患病率及自然史
Clin Infect Dis. 2009 Aug 15;49(4):522-8. doi: 10.1086/600882.
5
Strain-specific seroepidemiology and reinfection of cytomegalovirus.巨细胞病毒的菌株特异性血清流行病学及再感染
Microbes Infect. 2008 Oct;10(12-13):1363-9. doi: 10.1016/j.micinf.2008.08.001. Epub 2008 Aug 12.
6
Congenital cytomegalovirus infection in a highly seropositive semi-urban population in India.印度半城市高血清阳性人群中的先天性巨细胞病毒感染
Pediatr Infect Dis J. 2008 Sep;27(9):841-3. doi: 10.1097/INF.0b013e3181723d55.
7
Identification of a highly conserved region in the human cytomegalovirus glycoprotein H gene and design of molecular diagnostic methods targeting the region.人巨细胞病毒糖蛋白H基因中一个高度保守区域的鉴定及针对该区域的分子诊断方法的设计。
J Virol Methods. 2008 Jul;151(1):55-60. doi: 10.1016/j.jviromet.2008.03.022. Epub 2008 May 6.
8
Genetic variations in the gB, UL144 and UL149 genes of human cytomegalovirus strains collected from congenitally and postnatally infected Japanese children.从先天性和后天性感染的日本儿童中收集的人巨细胞病毒株gB、UL144和UL149基因的遗传变异。
Arch Virol. 2008;153(4):667-74. doi: 10.1007/s00705-008-0044-7. Epub 2008 Feb 14.
9
Association of the outcome of renal transplantation with antibody response to cytomegalovirus strain-specific glycoprotein H epitopes.肾移植结局与针对巨细胞病毒株特异性糖蛋白H表位的抗体反应的关联。
Clin Infect Dis. 2007 Jul 1;45(1):60-7. doi: 10.1086/518571. Epub 2007 May 23.
10
Hearing loss in children with congenital cytomegalovirus infection born to mothers with preexisting immunity.母亲具有既往免疫力的先天性巨细胞病毒感染患儿的听力损失
J Pediatr. 2006 Mar;148(3):332-6. doi: 10.1016/j.jpeds.2005.09.003.