Marine Natural Products Chemistry Laboratory, Korea Ocean Research & Development Institute , Ansan, P.O. Box 29, Seoul 425-600, Republic of Korea.
J Nat Prod. 2011 Dec 27;74(12):2582-7. doi: 10.1021/np200487k. Epub 2011 Dec 1.
Bioassay-guided isolation of bioactive metabolites from the ethyl acetate extract of a marine Bacillus sp. fermentation broth has led to the discovery of three new 24-membered macrolactones, macrolactins 1-3, which contain an oxetane, an epoxide, and a tetrahydropyran ring, respectively. The configurations of 1-3 were assigned by a combination of coupling constants, ROESY data analysis, and application of the modified Mosher's method. Compounds 1-3 showed in vitro antimicrobial activity.
生物活性代谢产物的生物测定指导从海洋芽孢杆菌发酵液的乙酸乙酯提取物中分离，发现了三个新的 24 元大环内酯，分别为大环内酯 1-3，它们分别含有一个氧杂环丁烷、一个环氧化物和一个四氢吡喃环。通过偶合常数、ROESY 数据分析以及改良的 Mosher 方法的应用，确定了 1-3 的构型。化合物 1-3 表现出体外抗菌活性。
