Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, 100091, China.
Forest Protection Station of Ordos, Ordos, 017000, China.
Arch Microbiol. 2021 Aug;203(6):3473-3481. doi: 10.1007/s00203-021-02323-5. Epub 2021 Apr 26.
L3-3HA, a Gram-negative-staining, facultatively anaerobic, motile bacterial strain, was isolated from the symptomatic bark of Salix matsudana canker in China. 16S rRNA gene analysis revealed that the novel strain shares the highest sequence similarity with Brenneria goodwinii FRB141 (95.5%). In phylogenetic trees based on four housekeeping genes (gyrB, rpoB, atpD, and infB) and the 16S rRNA gene sequence, the novel strain formed a separate branch from the five genera of the family Pectobacteriaceae (Lonsdalea, Brenneria, Dickeya, Pectobacterium, and Sodalis), suggesting that the novel strain should belong to a novel species of a novel genus within the family Pectobacteriaceae. The result was also supported by phylogenomics, amino acid identity and average nucleotide identity. The major fatty acids were C, C, C cyclo, and C cyclo ɷ8c. Genome analysis showed that the novel strain has a large genome (5.89 Mb) with 5,052 coding genes, including 181 virulence genes by searching the pathogen-host interactions database (PHI-base), indicating that the novel strain is a potential pathogen of plants and animals. Based on phenotypic and genotypic characteristics, the L3-3HA strain represents a novel species of a novel genus in the Pectobacteriaceae family, for which the name Affinibrenneria salicis gen nov. sp. nov. is proposed. The strain type is L3-3HA (= CFCC 15588 = LMG 31209).
L3-3HA 是一株革兰氏阴性、兼性厌氧、运动的细菌菌株，从中国柳树溃疡病的有症状树皮中分离得到。16S rRNA 基因分析表明，该新菌株与 Brenneria goodwinii FRB141（95.5%）具有最高的序列相似性。在基于四个管家基因（gyrB、rpoB、atpD 和 infB）和 16S rRNA 基因序列的系统发育树中，新菌株与 Pectobacteriaceae 科的五个属（Lonsdalea、Brenneria、Dickeya、Pectobacterium 和 Sodalis）形成了一个独立的分支，表明新菌株应属于 Pectobacteriaceae 科的一个新属的新种。这一结果也得到了系统基因组学、氨基酸同一性和平均核苷酸同一性的支持。主要脂肪酸为 C 16:0、C 18:1 cyclo、C 18:1 cyclo ω8c 和 C 18:0。基因组分析表明，该新菌株具有一个 5.89 Mb 的大基因组，包含 5052 个编码基因，通过搜索病原体-宿主相互作用数据库（PHI-base），其中包括 181 个毒力基因，表明该新菌株是动植物的潜在病原体。基于表型和基因型特征，L3-3HA 菌株代表 Pectobacteriaceae 科中的一个新属的新种，提议将其命名为 Affinibrenneria salicis gen. nov. sp. nov.。该菌株的模式株为 L3-3HA（= CFCC 15588 = LMG 31209）。
