细胞膜胆固醇是猪繁殖与呼吸综合征病毒进入和释放 MARC-145 细胞所必需的。
Cellular membrane cholesterol is required for porcine reproductive and respiratory syndrome virus entry and release in MARC-145 cells.
机构信息
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
出版信息
Sci China Life Sci. 2011 Nov;54(11):1011-8. doi: 10.1007/s11427-011-4236-0. Epub 2011 Dec 16.
Abstract
Cholesterol represents one of the key constituents of small, dynamic, sterol- and sphingolipid-enriched domains on the plasma membrane. It has been reported that many viruses depend on plasma membrane cholesterol for efficient infection. In this study, the role of the plasma membrane cholesterol in porcine reproductive and respiratory syndrome virus (PRRSV) infection of MARC-145 cells was investigated. Pretreatment of MARC-145 cells with methyl-β-cyclodextrin (MβCD), a drug used to deplete cholesterol from cellular membrane, significantly reduced PRRSV infection in a dose-dependent manner. This inhibition was partially reversed by supplementing exogenous cholesterol following MβCD treatment, suggesting that the inhibition of PRRSV infection was specifically mediated by removal of cellular cholesterol. Further detailed studies showed that depletion of cellular membrane cholesterol significantly inhibited virus entry, especially virus attachment and release. These results indicate that the presence of cholesterol in the cellular membrane is a key component of PRRSV infection.
摘要
胆固醇是质膜上小而动态的固醇和鞘脂富集域的关键成分之一。据报道，许多病毒依赖质膜胆固醇来实现有效的感染。在这项研究中，研究了质膜胆固醇在猪繁殖与呼吸综合征病毒 (PRRSV) 感染 MARC-145 细胞中的作用。用甲基-β-环糊精（MβCD）预处理 MARC-145 细胞，MβCD 是一种用于从细胞膜中去除胆固醇的药物，可剂量依赖性地显著降低 PRRSV 的感染。在用 MβCD 处理后补充外源性胆固醇，部分逆转了这种抑制作用，表明 PRRSV 感染的抑制是通过去除细胞胆固醇特异性介导的。进一步的详细研究表明，去除细胞膜胆固醇可显著抑制病毒进入，尤其是病毒附着和释放。这些结果表明，细胞膜中的胆固醇是 PRRSV 感染的关键组成部分。
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