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利用线粒体 nad6 作为鳞翅目种群变异性研究的补充标记。

Exploitation of mitochondrial nad6 as a complementary marker for studying population variability in Lepidoptera.

机构信息

Departamento de Entomologia e Acarologia, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brazil.

出版信息

Genet Mol Biol. 2011 Oct;34(4):719-25. doi: 10.1590/S1415-47572011000400028. Epub 2011 Oct 1.

DOI:10.1590/S1415-47572011000400028
PMID:22215980
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3229131/
Abstract

The applicability of mitochondrial nad6 sequences to studies of DNA and population variability in Lepidoptera was tested in four species of economically important moths and one of wild butterflies. The genetic information so obtained was compared to that of cox1 sequences for two species of Lepidoptera. nad6 primers appropriately amplified all the tested DNA targets, the generated data proving to be as informative and suitable in recovering population structures as that of cox1. The proposal is that, to obtain more robust results, this mitochondrial region can be complementarily used with other molecular sequences in studies of low level phylogeny and population genetics in Lepidoptera.

摘要

本研究以四种重要经济蛾类和一种野生蝴蝶为材料，检测了线粒体 nad6 序列在鳞翅目昆虫 DNA 和种群变异性研究中的适用性。所获得的遗传信息与两种鳞翅目昆虫的 cox1 序列进行了比较。nad6 引物适当地扩增了所有测试的 DNA 靶标，所产生的数据在恢复种群结构方面与 cox1 一样具有信息量和适用性。本研究提出，为了获得更稳健的结果，在研究鳞翅目昆虫的低水平系统发育和种群遗传学方面，可以将该线粒体区域与其他分子序列互补使用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ded/3229131/9dfe7ba6710c/gmb-34-1-719-gfig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ded/3229131/45fe8f45325b/gmb-34-1-719-gfig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ded/3229131/9dfe7ba6710c/gmb-34-1-719-gfig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ded/3229131/45fe8f45325b/gmb-34-1-719-gfig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ded/3229131/9dfe7ba6710c/gmb-34-1-719-gfig2.jpg

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