哺乳动物中广泛存在的 RNA 3'-末端寡聚尿苷酸化。
Widespread RNA 3'-end oligouridylation in mammals.
机构信息
Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, California 94143, USA.
出版信息
RNA. 2012 Mar;18(3):394-401. doi: 10.1261/rna.029306.111. Epub 2012 Jan 30.
Nontemplated 3'-end oligouridylation of RNA occurs in many species, including humans. Unlike the familiar phenomenon of polyadenylation, nontemplated addition of uridines to RNA is poorly characterized in higher eukaryotes. Recent studies have reported nontemplated 3'-end oligouridylation of small RNAs and mRNAs. Oligouridylation is involved in many aspects of microRNA biology from biogenesis to turnover of the mature species, and it may also mark long mRNAs for degradation by promoting decapping of the protective 5'-cap structure. To determine the prevalence of oligouridylation in higher eukaryotes, we used next-generation sequencing technology to deeply examine the population of small RNAs in human cells. Our data revealed widespread nontemplated nucleotide addition to the 3' ends of many classes of RNA, with short stretches of uridine being the most frequently added nucleotide.
RNA 的非模板 3' 端寡聚尿苷酸化发生在许多物种中，包括人类。与熟悉的聚腺苷酸化现象不同，高等真核生物中非模板尿苷酸的添加特征描述较差。最近的研究报道了小 RNA 和 mRNA 的非模板 3' 端寡聚尿苷酸化。寡聚尿苷酸化参与了 miRNA 生物学的许多方面，从生物发生到成熟体的周转，它还可以通过促进保护性 5' 帽结构的脱帽来标记长 mRNA 进行降解。为了确定寡聚尿苷酸化在高等真核生物中的普遍性，我们使用下一代测序技术深入研究了人类细胞中小 RNA 的群体。我们的数据显示，许多 RNA 类别的 3' 端广泛存在非模板核苷酸添加，其中尿嘧啶短片段是最常添加的核苷酸。
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