Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium.
Phytomedicine. 2012 Mar 15;19(5):409-12. doi: 10.1016/j.phymed.2011.10.005. Epub 2012 Feb 2.
Propionibacterium acnes is a Gram-positive bacterium that plays an important role in the pathogenesis of acne vulgaris. This organism is capable of biofilm formation and the decreased antimicrobial susceptibility of biofilm-associated cells may hamper efficient treatment. In addition, the prolonged use of systemic antibiotic therapy is likely to lead to the development and spread of antimicrobial resistance. In the present study we investigated whether P. acnes biofilms could be eradicated by plant extracts or their active compounds, and whether other mechanisms besides killing of biofilm cells could be involved. Out of 119 plant extracts investigated, we identified five with potent antibiofilm activity against P. acnes (extracts from Epimedium brevicornum, Malus pumila, Polygonum cuspidatum, Rhodiola crenulata and Dolichos lablab). We subsequently identified icariin, resveratrol and salidroside as active compounds in three of these extracts. Extracts from E. brevicornum and P. cuspidatum, as well as their active compounds (icariin and resveratrol, respectively) showed marked antibiofilm activity when used in subinhibitory concentrations, indicating that killing of microbial cells is not their only mode of action.
痤疮丙酸杆菌是一种革兰氏阳性细菌，在寻常痤疮的发病机制中起着重要作用。该生物能够形成生物膜，而生物膜相关细胞的抗菌敏感性降低可能会阻碍有效的治疗。此外，长期使用全身抗生素治疗可能导致抗菌药物耐药性的产生和传播。在本研究中，我们研究了植物提取物或其有效成分是否可以消除痤疮丙酸杆菌生物膜，以及是否涉及除杀死生物膜细胞以外的其他机制。在研究的 119 种植物提取物中，我们鉴定出 5 种对痤疮丙酸杆菌具有强大抗生物膜活性的提取物（来自淫羊藿、苹果、虎杖、红景天和菜豆的提取物）。随后，我们在其中 3 种提取物中鉴定出淫羊藿苷、白藜芦醇和红景天苷为有效化合物。淫羊藿和虎杖提取物及其有效成分（淫羊藿苷和白藜芦醇）在亚抑菌浓度下表现出明显的抗生物膜活性，表明杀死微生物细胞不是其唯一作用模式。
