Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan, China.
Anal Bioanal Chem. 2012 Jul;404(1):79-88. doi: 10.1007/s00216-012-6088-2. Epub 2012 Jun 1.
This paper reports the preparation of dapsone (DDS) imprinted polymer layer-coated silica submicron particles (SiO(2)) combined with chemiluminescence (CL) toward analysis of tracing DDS in practical samples. To induce the selective occurrence of surface polymerization, the amino groups were first grafted at the surface of SiO(2) by the (3-aminopropyl)triethoxysilane (APTES). The molecularly imprinted polymers (MIP) were coated at the surface of modified SiO(2) by the graft copolymerization. After the removal of templates, recognition sites of DDS were exposed in the polymer layers. The DDS-imprinted products were characterized by FT-IR, SEM, TEM, dynamic adsorption, and static adsorption tests. The proximity between the thickness of MIP layer and the spatial size of DDS indicated that the imprinted sites almost situated at the surface of MIP, leading to rapid adsorption saturation within 90 min. The apparent maximum binding amount of MIP toward DDS was evaluated as 14.98 mg·g(-1), which was much higher than that of non-molecularly imprinted polymers. The CL sensor provided a wide linear range for DDS within 1.0 × 10(-6) to 1.0 × 10(-4) mol·L(-1) with a detection limit of 5.27 × 10(-7) mol·L(-1) and the relative standard deviation of 1.8 % (n = 11) by determinations of 5.0 × 10(-6) mol·L(-1) DDS. This method was applied to determine DDS in urine samples and satisfactory results were obtained.
本文报道了载有磺胺（DDS）印迹聚合物层的二氧化硅亚微米颗粒（SiO2）的制备，结合化学发光（CL），用于分析实际样品中的痕量 DDS。为了诱导表面聚合的选择性发生，首先通过（3-氨丙基）三乙氧基硅烷（APTES）将氨基接枝到 SiO2 表面。通过接枝共聚将分子印迹聚合物（MIP）涂覆在改性 SiO2 的表面。模板去除后，DDS 的识别位点暴露在聚合物层中。通过 FT-IR、SEM、TEM、动态吸附和静态吸附试验对 DDS 印迹产物进行了表征。MIP 层的厚度与 DDS 的空间尺寸之间的接近表明，印迹位点几乎位于 MIP 的表面，导致在 90 分钟内快速达到吸附饱和。MIP 对 DDS 的表观最大结合量为 14.98mg·g-1，远高于非分子印迹聚合物。CL 传感器为 DDS 提供了 1.0×10-6 至 1.0×10-4 mol·L-1 的宽线性范围，检测限为 5.27×10-7 mol·L-1，5.0×10-6 mol·L-1 DDS 的测定相对标准偏差为 1.8%（n=11）。该方法用于测定尿样中的 DDS，取得了令人满意的结果。
