角膜共焦显微镜临床检查中的内皮样本量分析。

The endothelial sample size analysis in corneal specular microscopy clinical examinations.

机构信息

Department of Ophthalmology of Erast Gaertner Hospital and Federal University of Paraná, Curitiba, Brazil.

出版信息

Cornea. 2012 May;31(5):546-50. doi: 10.1097/ICO.0b013e3181cc7961.

DOI:10.1097/ICO.0b013e3181cc7961

PMID:22333658

Abstract

PURPOSE

To evaluate endothelial cell sample size and statistical error in corneal specular microscopy (CSM) examinations.

METHODS

One hundred twenty examinations were conducted with 4 types of corneal specular microscopes: 30 with each BioOptics, CSO, Konan, and Topcon corneal specular microscopes. All endothelial image data were analyzed by respective instrument software and also by the Cells Analyzer software with a method developed in our lab. A reliability degree (RD) of 95% and a relative error (RE) of 0.05 were used as cut-off values to analyze images of the counted endothelial cells called samples. The sample size mean was the number of cells evaluated on the images obtained with each device. Only examinations with RE < 0.05 were considered statistically correct and suitable for comparisons with future examinations. The Cells Analyzer software was used to calculate the RE and customized sample size for all examinations.

RESULTS

Bio-Optics: sample size, 97 ± 22 cells; RE, 6.52 ± 0.86; only 10% of the examinations had sufficient endothelial cell quantity (RE < 0.05); customized sample size, 162 ± 34 cells. CSO: sample size, 110 ± 20 cells; RE, 5.98 ± 0.98; only 16.6% of the examinations had sufficient endothelial cell quantity (RE < 0.05); customized sample size, 157 ± 45 cells. Konan: sample size, 80 ± 27 cells; RE, 10.6 ± 3.67; none of the examinations had sufficient endothelial cell quantity (RE > 0.05); customized sample size, 336 ± 131 cells. Topcon: sample size, 87 ± 17 cells; RE, 10.1 ± 2.52; none of the examinations had sufficient endothelial cell quantity (RE > 0.05); customized sample size, 382 ± 159 cells.

CONCLUSIONS

A very high number of CSM examinations had sample errors based on Cells Analyzer software. The endothelial sample size (examinations) needs to include more cells to be reliable and reproducible. The Cells Analyzer tutorial routine will be useful for CSM examination reliability and reproducibility.

摘要

目的

评估角膜共焦显微镜（CSM）检查中的内皮细胞样本量和统计误差。

方法

使用 4 种角膜共焦显微镜（BioOptics、CSO、Konan 和 Topcon 各 30 次）进行了 120 次检查。所有内皮细胞图像数据均由各自的仪器软件以及我们实验室开发的 Cells Analyzer 软件进行分析。使用可靠性度（RD）为 95%和相对误差（RE）为 0.05 作为截断值来分析称为样本的已计数内皮细胞的图像。样本量平均值是用每种设备获得的图像上评估的细胞数量。只有 RE < 0.05 的检查才被认为具有统计学意义，适合与未来的检查进行比较。Cells Analyzer 软件用于计算所有检查的 RE 和定制的样本量。

结果

Bio-Optics：样本量 97 ± 22 个细胞；RE 6.52 ± 0.86；只有 10%的检查具有足够的内皮细胞数量（RE < 0.05）；定制样本量 162 ± 34 个细胞。CSO：样本量 110 ± 20 个细胞；RE 5.98 ± 0.98；只有 16.6%的检查具有足够的内皮细胞数量（RE < 0.05）；定制样本量 157 ± 45 个细胞。Konan：样本量 80 ± 27 个细胞；RE 10.6 ± 3.67；没有一个检查具有足够的内皮细胞数量（RE > 0.05）；定制样本量 336 ± 131 个细胞。Topcon：样本量 87 ± 17 个细胞；RE 10.1 ± 2.52；没有一个检查具有足够的内皮细胞数量（RE > 0.05）；定制样本量 382 ± 159 个细胞。