利用下一代直接 DNA 测序技术检测临床标本中的可能生物恐怖主义剂弗朗西斯菌属。
Detection of a possible bioterrorism agent, Francisella sp., in a clinical specimen by use of next-generation direct DNA sequencing.
机构信息
Laboratory of Bacterial Genomics, Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan.
出版信息
J Clin Microbiol. 2012 May;50(5):1810-2. doi: 10.1128/JCM.06715-11. Epub 2012 Feb 15.
Abstract
Deep sequencing detected a potential bioterrorism agent, Francisella tularensis, in a human abscess sample (Iwaki-08) of unknown etiology. Identified single-nucleotide variations suggest that the Iwaki-08 case was associated with Francisella tularensis subsp. holarctica (biovar japonica) but not the highly virulent type A (Francisella tularensis subsp. tularensis).
摘要
深度测序在一个病因不明的人类脓肿样本（Iwaki-08）中检测到一种潜在的生物恐怖主义剂，即土拉弗朗西斯菌。鉴定出的单核苷酸变异表明，Iwaki-08 病例与土拉弗朗西斯菌亚种 holarctica（生物型日本型）有关，但与高毒力的 A 型（土拉弗朗西斯菌亚种 tularensis）无关。
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