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生物相容性纳米复合材料增强牙周膜干细胞的成骨和成牙骨质分化用于体外牙周组织再生

Biocompatible Nanocomposite Enhanced Osteogenic and Cementogenic Differentiation of Periodontal Ligament Stem Cells In Vitro for Periodontal Regeneration.

作者信息

Liu Jin, Dai Quan, Weir Michael D, Schneider Abraham, Zhang Charles, Hack Gary D, Oates Thomas W, Zhang Ke, Li Ang, Xu Hockin H K

机构信息

Key Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, China.

Clinical Research Center of Shannxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, China.

出版信息

Materials (Basel). 2020 Nov 4;13(21):4951. doi: 10.3390/ma13214951.

DOI:10.3390/ma13214951
PMID:33158111
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7663634/
Abstract

Decays in the roots of teeth is prevalent in seniors as people live longer and retain more of their teeth to an old age, especially in patients with periodontal disease and gingival recession. The objectives of this study were to develop a biocompatible nanocomposite with nano-sized calcium fluoride particles (Nano-CaF), and to investigate for the first time the effects on osteogenic and cementogenic induction of periodontal ligament stem cells (hPDLSCs) from human donors.Nano-CaF particles with a mean particle size of 53 nm were produced via a spray-drying machine.Nano-CaF was mingled into the composite at 0%, 10%, 15% and 20% by mass. Flexural strength (160 ± 10) MPa, elastic modulus (11.0 ± 0.5) GPa, and hardness (0.58 ± 0.03) GPa for Nano-CaF composite exceeded those of a commercial dental composite ( 0.05). Calcium (Ca) and fluoride (F) ions were released steadily from the composite. Osteogenic genes were elevated for hPDLSCs growing on 20% Nano-CaF. Alkaline phosphatase (ALP) peaked at 14 days. Collagen type 1 (COL1), runt-related transcription factor 2 (RUNX2) and osteopontin (OPN) peaked at 21 days. Cementogenic genes were also enhanced on 20% Nano-CaF composite, promoting cementum adherence protein (CAP), cementum protein 1 (CEMP1) and bone sialoprotein (BSP) expressions ( 0.05). At 7, 14 and 21 days, the ALP activity of hPDLSCs on 20% Nano-CaF composite was 57-fold, 78-fold, and 55-fold greater than those of control, respectively ( 0.05). Bone mineral secretion by hPDLSCs on 20% Nano-CaF composite was 2-fold that of control ( 0.05). In conclusion, the novel Nano-CaF composite was biocompatible and supported hPDLSCs. Nano-CaF composite is promising to fill tooth root cavities and release Ca and F ions to enhance osteogenic and cementogenic induction of hPDLSCs and promote periodontium regeneration.

摘要

随着人们寿命延长，老年时保留的牙齿增多，尤其是患有牙周病和牙龈退缩的患者，牙根龋坏在老年人中很常见。本研究的目的是开发一种含有纳米氟化钙颗粒（Nano-CaF）的生物相容性纳米复合材料，并首次研究其对人牙周膜干细胞（hPDLSCs）成骨诱导和牙骨质生成诱导的影响。通过喷雾干燥机制备了平均粒径为53 nm的Nano-CaF颗粒。将Nano-CaF按质量分数0%、10%、15%和20%混入复合材料中。Nano-CaF复合材料的弯曲强度（160±10）MPa、弹性模量（11.0±0.5）GPa和硬度（0.58±0.03）GPa均超过了商用牙科复合材料（P<0.05）。钙（Ca）和氟（F）离子从复合材料中稳定释放。在20% Nano-CaF上生长的hPDLSCs的成骨基因表达升高。碱性磷酸酶（ALP）在第14天达到峰值。I型胶原（COL1）、 runt相关转录因子2（RUNX2）和骨桥蛋白（OPN）在第21天达到峰值。在20% Nano-CaF复合材料上，牙骨质生成基因也增强，促进了牙骨质黏附蛋白（CAP）、牙骨质蛋白1（CEMP1）和骨唾液蛋白（BSP）的表达（P<0.05）。在第7、14和21天，20% Nano-CaF复合材料上hPDLSCs的ALP活性分别比对照组高57倍、78倍和55倍（P<0.05）。20% Nano-CaF复合材料上hPDLSCs的骨矿物质分泌是对照组的2倍（P<0.05）。总之，新型Nano-CaF复合材料具有生物相容性并支持hPDLSCs。Nano-CaF复合材料有望用于填充牙根龋洞，释放Ca和F离子，增强hPDLSCs的成骨和牙骨质生成诱导作用，促进牙周组织再生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec3f/7663634/eea9eae05295/materials-13-04951-g009.jpg
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