School of Metallurgy and Chemical Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, Jiangxi, China.
Biomed Environ Sci. 2011 Dec;24(6):688-93. doi: 10.3967/0895-3988.2011.06.015.
It is imperative to provide some consistent experimental results for the extraction of flavonid from Fructus Gardeniae.
The key extraction parameters that influenced the yield of flavonid from Fructus Gardeniae were optimized by employing an orthogonal experiment [L(9)(3)(4)], including the ratio of buffer solution (Na(2)B(4)O(7)·10H(2)O) to raw material, concentration of Fructus Gardeniae in extracting solution, extraction time and pH of buffer solution. An UV/Vis detector was used to perform the qualitative and quantitative analyses of the extracted flavonid with the using of the standard sample.
The maximum extraction yield of the crude extract was 5.0533 (mg/g) after 20 min when the mass ratio of Na(2)B(4)O(7)·10H(2)O to raw material was 0.4%, the concentration of Fructus Gardeniae in the extraction solution was 1/12 (g/mL), and pH of buffer solution was 4.5. The positive reactions to the Molish and HCl-Mg tests suggested that the extracted compound was flavonoid, and FTIR measurements also identified the presence of flavonoid in the extracts.
This work is expected to provide a basis for further research, development, and utilization of Fructus gardenia in flavonid extraction.
为从栀子中提取黄酮类化合物提供一些一致的实验结果是当务之急。
采用正交实验[L(9)(3)(4)]优化了影响栀子中黄酮类化合物产率的关键提取参数，包括缓冲液（硼砂）与原料的比例、提取溶液中栀子的浓度、提取时间和缓冲液的 pH 值。使用标准样品，通过紫外/可见检测器对提取的黄酮类化合物进行定性和定量分析。
当硼砂与原料的质量比为 0.4%、提取溶液中栀子的浓度为 1/12（g/mL）、缓冲液的 pH 值为 4.5 时，粗提物的最大提取产率为 5.0533（mg/g），为 20 min。Molish 和 HCl-Mg 反应呈阳性，表明提取的化合物为黄酮类化合物，FTIR 测量也表明提取物中存在黄酮类化合物。
这项工作有望为进一步研究、开发和利用栀子中的黄酮类化合物提取提供依据。
