State Key Laboratory of Natural Medicines, Nanjing 210009, China.
Talanta. 2012 Mar 15;91:7-17. doi: 10.1016/j.talanta.2012.01.039. Epub 2012 Jan 24.
Recent advances in monolithic systems, especially the development of functionalized monoliths, make it more tentative for separation science, such as enantiomer separation. Proteins, as one category of well-acknowledged chiral selectors, have won a reputation of separating a wide range of enantiomeric mixtures due to varieties of functional groups at their surface. However, the combination of protein and monoliths for chiral analysis is seldom reported, thus the state-of-the-art in monolith with proteins as chiral selectors for enantiomer separations is comprehensively reviewed. This review focuses on organic polymer monoliths, silica monoliths and molecularly imprinted polymer monoliths functionalized by proteins or glycoproteins, introducing the possible mechanisms, the immobilizing ways as well as the associated problems. Related areas such as partial filling technique are also covered.
整体式系统的最新进展，特别是功能化整体式的发展，使得分离科学（如对映体分离）更加可行。蛋白质作为一类公认的手性选择剂，由于其表面存在多种官能团，因此在分离各种对映体混合物方面享有盛誉。然而，蛋白质与整体式用于手性分析的组合很少有报道，因此，本文全面综述了以蛋白质作为手性选择剂用于对映体分离的整体式的最新进展。本文重点介绍了由蛋白质或糖蛋白功能化的有机聚合物整体式、硅胶整体式和分子印迹聚合物整体式，介绍了可能的机制、固定化方法以及相关问题。还涵盖了相关领域，如部分填充技术。
