Yang Na, Cui Xia, Qian Weifeng, Yu Shanshan, Liu Qun
China Agricultural University, Beijing, People's Republic of China.
Acta Vet Hung. 2012 Mar;60(1):83-92. doi: 10.1556/AVet.2012.007.
Abortion in dairy cattle causes considerable economic losses to the dairy industry. Aborted fetuses and samples from the corresponding aborting dams from 12 dairy herds in Beijing were tested for 9 abortifacient infectious pathogens by PCR between 2008 and 2010. From a total of 80 abortion cases collected during this period, infectious agents were detected in 45 (56.3%) cases, 22 (48.9%) of which represented co-infections with two or three infectious agents. The detected pathogens included infectious bovine rhinotracheitis virus (36.3%) and Neospora caninum (31.3%), followed by bovine viral diarrhoea virus (7.5%), Brucella abortus (6.3%), Tritrichomonas foetus (5%) and Toxoplasma gondii (1.3%). Campylobacter fetus, Coxiella burnetii and Chlamydophila psittaci were not detected in any abortion case. Findings from this study indicated that infectious bovine rhinotracheitis virus and Neospora caninum were the main potential causes of abortions in Beijing dairy herds, whereas the bacterial pathogens were not, in contrast to reports from other countries. This is the first study to test nine abortifacient infectious agents by PCR at the same time, and it is also the first time to report the involvement of a variety of infectious agents in bovine abortion cases in China.
奶牛流产给奶牛业造成了巨大的经济损失。2008年至2010年期间，对来自北京12个奶牛场的流产胎儿及相应流产母牛的样本进行了9种致流产传染性病原体的聚合酶链反应（PCR）检测。在此期间收集的总共80例流产病例中，在45例（56.3%）中检测到感染因子，其中22例（48.9%）为两种或三种感染因子的合并感染。检测到的病原体包括牛传染性鼻气管炎病毒（36.3%）和犬新孢子虫（31.3%），其次是牛病毒性腹泻病毒（7.5%）、牛布鲁氏菌（6.3%）、胎儿三毛滴虫（5%）和弓形虫（1.3%）。在任何流产病例中均未检测到胎儿弯曲杆菌、伯氏考克斯体和鹦鹉热衣原体。本研究结果表明，牛传染性鼻气管炎病毒和犬新孢子虫是北京奶牛场流产的主要潜在原因，而细菌病原体并非如此，这与其他国家的报道不同。这是首次同时通过PCR检测9种致流产传染性病原体的研究，也是首次在中国报道多种感染因子与牛流产病例有关。
