Weiss N, Delius M, Gambihler S, Dirschedl P, Goetz A, Brendel W
Institute for Surgical Research, University of Munich, Federal Republic of Germany.
Ultrasound Med Biol. 1990;16(6):595-605. doi: 10.1016/0301-5629(90)90025-8.
We examined the influence of different shock wave application modes with a Dornier XL1 electrohydraulic lithotripter on the growth of A-Mel 3 and SSK2 tumors implanted under the dorsal skin of hamsters or mice. In a basic protocol, 500 shock waves a day on 4 consecutive days were administered at a discharge rate of 100 waves per minute and focused to the tumor center. This did not affect A-Mel 3 growth. A similar result was obtained with the basic protocol modified to 1000 shock waves a day and a wave application rate of 100 shock waves per second. Growth of A-Mel 3 and SSK2 tumors was significantly delayed, when the basic protocol was used, but the 500 shock waves a day were distributed over four points at the tumor edges and the tumor center. With the same shock wave protocol, lowering the water level over the tumor from 10 cm to 1 cm induced temporary regressions of SSK2 tumors. This was not due to the higher energy applied to the tumor, since twice the number of shock waves (1000 a day instead of 500 a day) was applied at a high water level and did not induce regressions. Four consecutive treatments with intervals between treatments shortened to 3 h and an additional treatment 12 h later at a low water level completely controlled tumor growth in 8 out of 12 SSK2 tumors for more than 150 days. The result showed that addition of a reflected wave from the water surface was most important for the shock wave effect, and suggested that shock wave devices generating similar wave forms should be applied for tumor therapy.
我们使用多尼尔XL1型液电碎石机研究了不同冲击波应用模式对植入仓鼠或小鼠背部皮肤下的A-Mel 3和SSK2肿瘤生长的影响。在一个基本方案中，以每分钟100次冲击波的放电速率，连续4天每天施加500次冲击波，并聚焦于肿瘤中心。这并未影响A-Mel 3的生长。将基本方案修改为每天1000次冲击波且冲击波应用速率为每秒100次时，也得到了类似结果。当使用基本方案，但每天500次冲击波分布在肿瘤边缘和肿瘤中心的四个点时，A-Mel 3和SSK2肿瘤的生长显著延迟。采用相同的冲击波方案，将肿瘤上方的水位从10厘米降至1厘米可使SSK2肿瘤暂时消退。这并非由于施加于肿瘤的能量更高，因为在高水位时施加了两倍数量的冲击波（每天1000次而非500次），但并未引起肿瘤消退。连续进行四次治疗，治疗间隔缩短至3小时，并在12小时后于低水位进行额外一次治疗，12只SSK2肿瘤中有8只的肿瘤生长被完全控制超过150天。结果表明，水面反射波的加入对冲击波效应最为重要，并提示应将产生类似波形的冲击波装置应用于肿瘤治疗。
