CRA-UTV, Research Unit for Viticulture and Enology in Southern Italy, Turi, BA, Italy.
Mol Biotechnol. 2013 Mar;53(3):278-88. doi: 10.1007/s12033-012-9524-9.
Two different hypothesis for the parentage of 'Sangiovese', the most important and widespread Italian winegrape, have been proposed by some previous studies. We screened our grapevine collection, mostly comprising south Italian cultivars collected to preserve biodiversity, to asses kinships. Surprisingly we found two previously unreported candidate parents for 'Sangiovese'. The first putative parent is 'Ciliegiolo' a well know variety already addressed as relative of 'Sangiovese'; the second putative parent is 'Negrodolce', an old local variety we recovered and was considered lost during the last century. In order to obtain a stronger statistical support for this new kinship, we tested seventy different microsatellite markers but only 57 were found reliable. The new proposed parentage stood well even with such a in depth molecular analysis whereas only one discrepancy was found in one of the 57 microsatellite marker analyzed. This discrepancy is certainly due to a null-allele and therefore it should not impair our hypothesis but it points out limits of the microsatellites profiling as a pedigree research method considering that this is the third different kinship proposed so far for 'Sangiovese'. Thus in this article, by means of detailed molecular fingerprinting, we provide a completely new strong evidence for a south Italian origin of 'Sangiovese' and we discuss our findings comparing our data with those previously reported by other authors.
两个不同的假说对 '桑娇维塞'的起源，最重要和最广泛的意大利葡萄品种，已被一些以前的研究提出。我们筛选了我们的葡萄收集，主要包括南意大利品种收集以保护生物多样性，以评估亲属关系。令人惊讶的是，我们发现了两个以前未报告的 '桑娇维塞'候选父母。第一个假定的亲本是 'Ciliegiolo'，这是一个已经被认为是 '桑娇维塞'相关的知名品种；第二个假定的亲本是 'Negrodolce'，是我们重新发现的一种古老的本地品种，在上个世纪被认为已经失传。为了获得对这种新亲缘关系更强的统计支持，我们测试了 70 个不同的微卫星标记，但只有 57 个被认为是可靠的。即使进行了如此深入的分子分析，新提出的亲缘关系仍然成立，而在分析的 57 个微卫星标记中只有一个存在差异。这种差异肯定是由于无效等位基因造成的，因此不应影响我们的假设，但它指出了微卫星图谱作为系谱研究方法的局限性，考虑到到目前为止，这是第三个不同的亲缘关系被提出的 '桑娇维塞'。因此，在本文中，通过详细的分子指纹分析，我们提供了一个全新的、强有力的证据，证明 '桑娇维塞'起源于意大利南部，并将我们的发现与其他作者以前报道的数据进行了比较。
