鉴定非洲油桃木的抗增殖活性。
Characterization of the antiproliferative activity of Xylopia aethiopica.
机构信息
Université de Toulouse; ITAV-UMS3039, F-31106 Toulouse, France.
出版信息
Cell Div. 2012 Mar 12;7(1):8. doi: 10.1186/1747-1028-7-8.
BACKGROUND
Xylopia aethiopica, a plant found throughout West Africa, has both nutritional and medicinal uses. The present study aims to characterize the effects of extracts of this plant on cancer cells.
RESULTS
We report that X. aethiopica extract prepared with 70% ethanol has antiproliferative activity against a panel of cancer cell lines. The IC50 was estimated at 12 μg/ml against HCT116 colon cancer cells, 7.5 μg/ml and > 25 μg/ml against U937 and KG1a leukemia cells, respectively. Upon fractionation of the extract by HPLC, the active fraction induced DNA damage, cell cycle arrest in G1 phase and apoptotic cell death. By using NMR and mass spectrometry, we determined the structure of the active natural product in the HPLC fraction as ent-15-oxokaur-16-en-19-oic acid.
CONCLUSION
The main cytotoxic and DNA-damaging compound in ethanolic extracts of Xylopia aethiopica is ent-15-oxokaur-16-en-19-oic acid.
背景
在整个西非都有发现的植物黄皮树，具有营养和药用两种用途。本研究旨在研究该植物提取物对癌细胞的作用。
结果
我们报告称，用 70%乙醇制备的黄皮树提取物对一系列癌细胞系具有抗增殖活性。IC50 值估计为 12μg/ml 对 HCT116 结肠癌细胞，分别为 7.5μg/ml 和 >25μg/ml 对 U937 和 KG1a 白血病细胞。通过 HPLC 对提取物进行分段后，活性部分诱导 DNA 损伤、G1 期细胞周期停滞和凋亡细胞死亡。通过使用 NMR 和质谱，我们确定了在 HPLC 馏分中活性天然产物的结构为 ent-15-氧代-16-开-19-酸。
结论
Xylopia aethiopica 乙醇提取物中的主要细胞毒性和 DNA 损伤化合物是 ent-15-氧代-16-开-19-酸。
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