Faculty of Medicine, University of Khartoum, P,O, Box 102, Khartoum, Sudan.
Diagn Pathol. 2012 Mar 21;7:30. doi: 10.1186/1746-1596-7-30.
Assessment of hemoglobin is one of the most reliable indicators for anemia, and is widely used to screen for anemia among pregnant women. The HemoCue® has been widely used for as a point-of-care device for hemoglobin estimation in health facilities. Previous studies showed contradictory results regarding the accuracy of HemoCue®.
This was a hospital-based cross sectional study carried- out among pregnant women at Khartoum hospital in Sudan to find out whether the measurement of hemoglobin concentration by HemoCue® using venous or capillary samples was comparable to that of the automated hematology analyzer as standard. Bland and Altman method was used to compare the measurements with an acceptable difference of ± 1.0 g/dl.
Among the 108 subjects in this study the mean (SD) level of hemoglobin level using HemoCue® venous sample, HemoCue® capillary sample and automated hematology analyzer were 12.70 (1.77), 12.87 (2.04) and 11.53 (1.63) g/dl, respectively. Although the correlations between the measurements were all significant there was no agreement between HemoCue® and automated hematology analyzer. The bias + SD (limits of agreement) for HemoCue® venous versus hematology analyzer was 1.17 ± 1.57 (-1.97, 4.31) g/dl, HemoCue® capillary versus hematology analyzer was 1.34 ± 1.85 (-2.36, 5.04) g/dl, and HemoCue® venous versus HemoCue® capillary samples was 017 ± 1.90 and (3.97-3.63) g/dl.
Hemoglobin concentration assessment by HemoCue® using either venous or capillary blood samples has shown unacceptable agreement with automated hematology analyzer.
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血红蛋白评估是贫血最可靠的指标之一，广泛用于孕妇贫血筛查。HemoCue®已广泛用于医疗机构的血红蛋白即时检测。先前的研究表明 HemoCue®的准确性存在矛盾的结果。
这是一项在苏丹喀土穆医院进行的基于医院的横断面研究，旨在确定使用 HemoCue®静脉或毛细血管样本测量血红蛋白浓度是否与自动化血液分析仪作为标准相媲美。采用 Bland 和 Altman 方法比较测量值，可接受的差异为±1.0 g/dl。
在这项研究的 108 名受试者中，HemoCue®静脉样本、HemoCue®毛细血管样本和自动化血液分析仪的平均（SD）血红蛋白水平分别为 12.70（1.77）、12.87（2.04）和 11.53（1.63）g/dl。虽然测量值之间的相关性均具有统计学意义，但 HemoCue®与自动化血液分析仪之间没有一致性。HemoCue®静脉与血液分析仪的偏差+SD（一致性界限）为 1.17±1.57（-1.97，4.31）g/dl，HemoCue®毛细血管与血液分析仪的偏差+SD 为 1.34±1.85（-2.36，5.04）g/dl，HemoCue®静脉与 HemoCue®毛细血管样本的偏差+SD 为 017±1.90（3.97-3.63）g/dl。
使用 HemoCue®静脉或毛细血管血样评估血红蛋白浓度与自动化血液分析仪的一致性不可接受。
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