Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, USA.
J Virol. 2012 Jun;86(12):6427-33. doi: 10.1128/JVI.00341-12. Epub 2012 Apr 4.
We isolated stem/progenitor epithelial cells from the lungs of 4- to 6-week-old pigs. The epithelial progenitor colony cells were surrounded by mesenchymal stromal cells. The progenitor epithelial colony cells expressed stem cell markers such as octamer binding transcription factor 4 (Oct4) and stage-specific embryonic antigen 1 (SSEA-1), as well as the epithelial markers pancytokeratin, cytokeratin-18, and occludin, but not mesenchymal (CD44, CD29, and CD90) and hematopoietic (CD45) markers. The colony cells had extensive self-renewal potential and had the capacity to undergo differentiation to alveolar type I- and type II-like pneumocytes. Additionally, these cells expressed sialic acid receptors and supported the active replication of influenza virus, which was accompanied by cell lysis. The lysis of progenitor epithelial cells by influenza virus may cause a marked reduction in the potential of progenitor cells for self renewal and for their ability to differentiate into specialized cells of the lung. These observations suggest the possible involvement of lung stem/progenitor cells in influenza virus infection.
我们从 4 至 6 周龄猪的肺部分离出干细胞/祖细胞上皮细胞。上皮祖细胞集落细胞被间质基质细胞包围。祖细胞上皮集落细胞表达干细胞标志物，如八聚体结合转录因子 4（Oct4）和阶段特异性胚胎抗原 1（SSEA-1），以及上皮标志物广谱细胞角蛋白、细胞角蛋白-18 和紧密连接蛋白，但不表达间充质（CD44、CD29 和 CD90）和造血（CD45）标志物。集落细胞具有广泛的自我更新潜力，并具有分化为肺泡 I 型和 II 型样肺细胞的能力。此外，这些细胞表达唾液酸受体，并支持流感病毒的复制，同时伴有细胞裂解。流感病毒对祖细胞上皮细胞的裂解可能导致祖细胞自我更新的潜力和分化为肺特化细胞的能力显著降低。这些观察结果表明，肺干细胞/祖细胞可能参与了流感病毒感染。
