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用于检测中国湖南省断奶仔猪中猪萨波病毒的逆转录聚合酶链反应试验。

RT-PCR test for detecting porcine sapovirus in weanling piglets in Hunan Province, China.

作者信息

Liu Guo-Hua, Li Run-Cheng, Huang Ze-Bin, Yang Jun, Xiao Chao-Ting, Li Jing, Li Man-Xiang, Yan Yun-Qiu, Yu Xing-Long

机构信息

College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province, 410128, People's Republic of China.

出版信息

Trop Anim Health Prod. 2012 Oct;44(7):1335-9. doi: 10.1007/s11250-012-0138-9. Epub 2012 Apr 11.

DOI:10.1007/s11250-012-0138-9
PMID:22492394
Abstract

The prevalence of porcine sapovirus infection in weanling pigs was investigated in Hunan Province, China, between August 2006 and October 2007. A total of 153 diarrheic fecal samples from ten intensive pig farms from ten representative administrative regions in Hunan province were examined for porcine sapoviruses using RT-PCR. Twenty-two of 153 (14.37 %) samples were found to contain porcine sapoviruses. Phylogenetic analysis showed that all the porcine sapovirus isolates in Hunan Province belonged to the porcine sapovirus genogroup III. The results of the present investigation have implications for the control of porcine sapovirus infection in pigs in Hunan Province, China.

摘要

2006年8月至2007年10月期间,在中国湖南省对断奶仔猪中猪萨波病毒感染的流行情况进行了调查。使用逆转录聚合酶链反应(RT-PCR)对来自湖南省十个代表性行政区的十个集约化养猪场的总共153份腹泻粪便样本进行了猪萨波病毒检测。在153份样本中有22份(14.37%)被发现含有猪萨波病毒。系统发育分析表明,湖南省所有猪萨波病毒分离株均属于猪萨波病毒基因组III。本调查结果对中国湖南省猪的猪萨波病毒感染防控具有重要意义。

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