Cho Sung In, Nam Yun Sung, Chu Li Ying, Lee Jong Hyuk, Bang Joon Seok, Kim Hye Ryoun, Kim Hyoung-Chun, Lee Yu Jeung, Kim Hyeong-Dong, Sul Jung Duk, Kim Daejin, Chung Yoon Hee, Jeong Ji Hoon
Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, Korea.
Bioelectromagnetics. 2012 Oct;33(7):568-74. doi: 10.1002/bem.21715. Epub 2012 Apr 11.
Our previous study has shown that an extremely low-frequency magnetic field (ELF-MF) induces nitric oxide (NO) synthesis by Ca(2+) -dependent NO synthase (NOS) in rat brain. The present study was designed to confirm that ELF-MF affects neuronal NOS (nNOS) in several brain regions and to investigate the correlation between NO and nNOS activation. The exposure of rats to a 2 mT, 60 Hz ELF-MF for 5 days resulted in increases of NO levels in parallel with cGMP elevations in the cerebral cortex, striatum, and hippocampus. Cresyl violet staining and electron microscopic evaluation revealed that there were no significant differences in the morphology and number of neurons in the cerebral cortex, striatum, and hippocampus. Differently, the numbers of nNOS-immunoreactive (IR) neurons were significantly increased in those cerebral areas in ELF-MF-exposed rats. These data suggest that the increase in NO could be due to the increased expression and activation of nNOS in cells. Based on NO signaling in physiological and pathological states, ELF-MF created by electric power systems may induce various physiological changes in modern life.
我们之前的研究表明，极低频磁场（ELF-MF）可通过大鼠脑中依赖Ca(2+)的一氧化氮合酶（NOS）诱导一氧化氮（NO）的合成。本研究旨在证实ELF-MF对多个脑区的神经元型NOS（nNOS）有影响，并研究NO与nNOS激活之间的相关性。将大鼠暴露于2 mT、60 Hz的ELF-MF中5天，导致大脑皮层、纹状体和海马体中NO水平升高，同时cGMP也升高。甲酚紫染色和电子显微镜评估显示，大脑皮层、纹状体和海马体中神经元的形态和数量没有显著差异。不同的是，在暴露于ELF-MF的大鼠的这些脑区中，nNOS免疫反应性（IR）神经元的数量显著增加。这些数据表明，NO的增加可能是由于细胞中nNOS表达和激活的增加。基于生理和病理状态下的NO信号传导，电力系统产生的ELF-MF可能在现代生活中诱导各种生理变化。
