Farivar Taghi Naserpour, Johari Pouran, Shafei Shilan, Najafipour Reza
Cell and Molecular Research Center, Qazvin University of Medical Sciences, Qavzin, Iran.
Asian Pac J Cancer Prev. 2012;13(1):339-42. doi: 10.7314/apjcp.2012.13.1.339.
About one third of the human population suffer cancer during their lifetime and more than 20% of total morbidity is related to neoplasia. Cervical cancer is generally the most common cancer in developing countries and the second most common in women globally. The role of human papilloma viruses viruses in its induction is clear. However, the involvement of herpes simplex virus type 2 (HSV-2) is controversial. Therefore a survey was conducted of the prevalence of HSV-2 in patients with cervical cancer and also healthy people with sensitive and quantitative Taq Man real-time PCR assay.
Seventy six formaldehyde fixed paraffin embedded tissue specimens from patients with histologically proven history of cervical cancer as well as 150 control blocks were sectioned for deparaffinization and DNA extraction.
There was no HSV-2 DNA in our patient specimens but four control samples were positive, all with a history of hysterectomy.
Considering the absence of any positive viral HSV-2 DNA in our patients and also the presence of four positive specimens among our controls, we did not find any relationship between the presence of HSV-2 DNA and cervical cancer.
约三分之一的人类在其一生中会患癌症，总发病率的20%以上与肿瘤形成有关。宫颈癌通常是发展中国家最常见的癌症，在全球女性中是第二常见的癌症。人乳头瘤病毒在其诱发过程中的作用是明确的。然而，2型单纯疱疹病毒（HSV-2）的参与存在争议。因此，采用灵敏且定量的Taq Man实时聚合酶链反应分析法，对宫颈癌患者以及健康人群中HSV-2的流行情况进行了一项调查。
将76例经组织学证实有宫颈癌病史患者的甲醛固定石蜡包埋组织标本以及150个对照样本进行切片，以进行脱石蜡处理和DNA提取。
我们的患者标本中未检测到HSV-2 DNA，但有4个对照样本呈阳性，所有阳性对照样本均有子宫切除史。
鉴于我们的患者中未发现任何阳性的病毒HSV-2 DNA，且我们的对照中有4个阳性标本，我们未发现HSV-2 DNA的存在与宫颈癌之间存在任何关联。
