脂滴的蛋白质组学：从细菌到人都保守的细胞器的结构、动态和功能。
The proteomics of lipid droplets: structure, dynamics, and functions of the organelle conserved from bacteria to humans.
机构信息
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
出版信息
J Lipid Res. 2012 Jul;53(7):1245-53. doi: 10.1194/jlr.R024117. Epub 2012 Apr 25.
Abstract
Lipid droplets are cellular organelles that consists of a neutral lipid core covered by a monolayer of phospholipids and many proteins. They are thought to function in the storage, transport, and metabolism of lipids, in signaling, and as a specialized microenvironment for metabolism in most types of cells from prokaryotic to eukaryotic organisms. Lipid droplets have received a lot of attention in the last 10 years as they are linked to the progression of many metabolic diseases and hold great potential for the development of neutral lipid-derived products, such as biofuels, food supplements, hormones, and medicines. Proteomic analysis of lipid droplets has yielded a comprehensive catalog of lipid droplet proteins, shedding light on the function of this organelle and providing evidence that its function is conserved from bacteria to man. This review summarizes many of the proteomic studies on lipid droplets from a wide range of organisms, providing an evolutionary perspective on this organelle.
摘要
脂滴是由中性脂质核心被一层磷脂和许多蛋白质覆盖组成的细胞细胞器。它们被认为在脂质的储存、运输和代谢、信号传递以及作为大多数类型的从原核生物到真核生物的细胞中的代谢的特殊微环境中发挥作用。脂滴在过去的 10 年中受到了很多关注，因为它们与许多代谢疾病的进展有关，并为中性脂质衍生产品的开发提供了很大的潜力，如生物燃料、食品补充剂、激素和药物。对脂滴的蛋白质组学分析产生了脂滴蛋白的综合目录，阐明了这个细胞器的功能，并提供了证据表明其功能从细菌到人类都是保守的。这篇综述总结了来自广泛生物体的许多关于脂滴的蛋白质组学研究，为这个细胞器提供了一个进化的视角。
相似文献
1
The proteomics of lipid droplets: structure, dynamics, and functions of the organelle conserved from bacteria to humans.脂滴的蛋白质组学：从细菌到人都保守的细胞器的结构、动态和功能。
J Lipid Res. 2012 Jul;53(7):1245-53. doi: 10.1194/jlr.R024117. Epub 2012 Apr 25.
2
Proteomic studies of isolated lipid droplets from bacteria, C. elegans, and mammals.对来自细菌、秀丽隐杆线虫和哺乳动物的分离脂滴进行的蛋白质组学研究。
Methods Cell Biol. 2013;116:1-14. doi: 10.1016/B978-0-12-408051-5.00001-2.
3
A role for lipid droplets in inter-membrane lipid traffic.脂滴在膜间脂质运输中的作用。
Proteomics. 2009 Feb;9(4):914-21. doi: 10.1002/pmic.200800584.
4
The contribution of the Drosophila model to lipid droplet research.果蝇模型在脂滴研究中的贡献。
Prog Lipid Res. 2011 Oct;50(4):348-56. doi: 10.1016/j.plipres.2011.04.001. Epub 2011 May 17.
5
Lipidomics reveals that adiposomes store ether lipids and mediate phospholipid traffic.脂质组学研究表明，脂肪体储存醚脂并介导磷脂运输。
J Lipid Res. 2007 Apr;48(4):837-47. doi: 10.1194/jlr.M600413-JLR200. Epub 2007 Jan 8.
6
Dynamic activity of lipid droplets: protein phosphorylation and GTP-mediated protein translocation.脂滴的动态活动：蛋白质磷酸化与GTP介导的蛋白质易位
J Proteome Res. 2007 Aug;6(8):3256-65. doi: 10.1021/pr070158j. Epub 2007 Jul 3.
7
Lipid droplets: a classic organelle with new outfits.脂滴：一个拥有新“外衣”的经典细胞器。
Histochem Cell Biol. 2008 Aug;130(2):263-79. doi: 10.1007/s00418-008-0449-0. Epub 2008 Jun 11.
8
Isolating lipid droplets from multiple species.从多种物种中分离脂滴。
Nat Protoc. 2013 Jan;8(1):43-51. doi: 10.1038/nprot.2012.142. Epub 2012 Dec 6.
9
Lipid droplets and their interactions with other organelles in liver diseases.肝疾病中的脂滴及其与其他细胞器的相互作用。
Int J Biochem Cell Biol. 2021 Apr;133:105937. doi: 10.1016/j.biocel.2021.105937. Epub 2021 Jan 30.
10
Single cell synchrotron FT-IR microspectroscopy reveals a link between neutral lipid and storage carbohydrate fluxes in S. cerevisiae.单细胞同步辐射傅里叶变换红外显微镜光谱学揭示了酿酒酵母中中性脂质和储存碳水化合物通量之间的联系。
PLoS One. 2013 Sep 11;8(9):e74421. doi: 10.1371/journal.pone.0074421. eCollection 2013.
引用本文的文献
1
Emerging role of lipid droplets in obscure puffer immune response against .脂滴在河豚针对……的免疫反应中的新作用 。
你提供的原文似乎不完整，“against”后面缺少具体内容。
Mar Life Sci Technol. 2025 Mar 17;7(2):271-283. doi: 10.1007/s42995-025-00286-w. eCollection 2025 May.
2
LIPID DROPLET PROTEIN OF SEEDS is involved in the control of lipid droplet size in Arabidopsis seeds and seedlings.种子脂滴蛋白参与拟南芥种子和幼苗中脂滴大小的调控。
Plant Cell. 2025 May 9;37(5). doi: 10.1093/plcell/koaf121.
3
Cofilin(s) and Mitochondria: Function Beyond Actin Dynamics.丝切蛋白与线粒体：肌动蛋白动力学之外的功能
Int J Mol Sci. 2025 Apr 25;26(9):4094. doi: 10.3390/ijms26094094.
4
Omics Approaches to Study Perilipins and Their Significant Biological Role in Cardiometabolic Disorders.用于研究 perilipins 及其在心脏代谢紊乱中重要生物学作用的组学方法。
Int J Mol Sci. 2025 Jan 10;26(2):557. doi: 10.3390/ijms26020557.
5
Lipid accumulation in adipose tissue-resident iNKT cells contributes to an inflammatory phenotype.脂肪组织驻留型 iNKT 细胞中的脂质堆积导致炎症表型。
Adipocyte. 2024 Dec;13(1):2421750. doi: 10.1080/21623945.2024.2421750. Epub 2024 Nov 1.
6
Lipase activated endocytosis-like behavior of oil-in-water emulsion.油包水乳状液的脂酶激活型内吞样作用
Nat Commun. 2024 Oct 2;15(1):8517. doi: 10.1038/s41467-024-52802-8.
7
HIV-TAT dysregulates microglial lipid metabolism through SREBP2/miR-124 axis: Implication of lipid droplet accumulation microglia in NeuroHIV.HIV-TAT通过SREBP2/miR-124轴失调小胶质细胞脂质代谢：脂滴在神经HIV中的积累对小胶质细胞的影响
Brain Behav Immun. 2025 Jan;123:108-122. doi: 10.1016/j.bbi.2024.09.011. Epub 2024 Sep 10.
8
Theory of Lipid Metabolism Disorders in Rhinitis and Asthma (Lipid Droplets).鼻炎和哮喘中的脂质代谢紊乱理论（脂滴）
Cell Biochem Biophys. 2025 Mar;83(1):25-31. doi: 10.1007/s12013-024-01469-5. Epub 2024 Aug 4.
9
Cellular communication through extracellular vesicles and lipid droplets.通过细胞外囊泡和脂滴进行细胞通讯。
J Extracell Biol. 2023 Mar 1;2(3):e77. doi: 10.1002/jex2.77. eCollection 2023 Mar.
10
Rab1b facilitates lipid droplet growth by ER-to-lipid droplet targeting of DGAT2.Rab1b 通过将 DGAT2 靶向到脂滴来促进脂滴生长。
Sci Adv. 2024 May 31;10(22):eade7753. doi: 10.1126/sciadv.ade7753. Epub 2024 May 29.
本文引用的文献
1
Biochemical characterization of RNase HII from Aeropyrum pernix.嗜热古菌 Aeropyrum pernix 的核糖核酸酶 HII 的生化特性研究。
Acta Biochim Biophys Sin (Shanghai). 2012 Apr;44(4):339-46. doi: 10.1093/abbs/gms011. Epub 2012 Feb 23.
2
Characterization of the lipid droplet proteome of a clonal insulin-producing β-cell line (INS-1 832/13).鉴定克隆胰岛素分泌细胞系（INS-1 832/13）的脂滴蛋白质组。
J Proteome Res. 2012 Feb 3;11(2):1264-73. doi: 10.1021/pr200957p. Epub 2012 Jan 24.
3
Developmental changes in the protein composition of Manduca sexta lipid droplets.曼陀罗蚕脂滴蛋白质组成的发育变化。
Insect Biochem Mol Biol. 2012 May;42(5):305-20. doi: 10.1016/j.ibmb.2012.01.001. Epub 2012 Jan 5.
4
Identification of the major functional proteins of prokaryotic lipid droplets.鉴定原核类脂滴的主要功能蛋白。
J Lipid Res. 2012 Mar;53(3):399-411. doi: 10.1194/jlr.M021899. Epub 2011 Dec 15.
5
Gene cloning and characterization of a cold-adapted β-glucosidase belonging to glycosyl hydrolase family 1 from a psychrotolerant bacterium Micrococcus antarcticus.南极微球菌中属于糖苷水解酶家族 1 的冷适应β-葡萄糖苷酶的基因克隆与特性分析。
Enzyme Microb Technol. 2011 Jun 10;49(1):94-9. doi: 10.1016/j.enzmictec.2011.03.001. Epub 2011 Mar 21.
6
Proteomic profiling of oil bodies isolated from the unicellular green microalga Chlamydomonas reinhardtii: with focus on proteins involved in lipid metabolism.从单细胞绿藻莱茵衣藻中分离的油体的蛋白质组学分析：重点是参与脂质代谢的蛋白质。
Proteomics. 2011 Nov;11(21):4266-73. doi: 10.1002/pmic.201100114. Epub 2011 Sep 19.
7
Proteome of skeletal muscle lipid droplet reveals association with mitochondria and apolipoprotein a-I.骨骼肌脂滴蛋白质组与线粒体和载脂蛋白 a-I 相关。
J Proteome Res. 2011 Oct 7;10(10):4757-68. doi: 10.1021/pr200553c. Epub 2011 Sep 15.
8
Lipid particles/droplets of the yeast Saccharomyces cerevisiae revisited: lipidome meets proteome.重新审视酿酒酵母的脂质颗粒/液滴：脂质组与蛋白质组的相遇
Biochim Biophys Acta. 2011 Dec;1811(12):1165-76. doi: 10.1016/j.bbalip.2011.07.015. Epub 2011 Jul 26.
9
Oil body proteins sequentially accumulate throughout seed development in Brassica napus.油体蛋白在油菜籽的整个发育过程中依次积累。
J Plant Physiol. 2011 Nov 15;168(17):2015-20. doi: 10.1016/j.jplph.2011.06.007. Epub 2011 Jul 31.
10
Lipid bodies in coral-dinoflagellate endosymbiosis: proteomic and ultrastructural studies.珊瑚-虫黄藻共生体中的脂滴：蛋白质组学和超微结构研究。
Proteomics. 2011 Sep;11(17):3540-55. doi: 10.1002/pmic.201000552. Epub 2011 Jul 27.
Zotero 插件