Division of Polar Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 406-840, Republic of Korea.
School of Biological Sciences, College of Natural Science, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-747, Republic of Korea.
Int J Syst Evol Microbiol. 2015 Jan;65(Pt 1):183-188. doi: 10.1099/ijs.0.069740-0. Epub 2014 Oct 14.
A Gram-staining-negative, yellow-pigmented, aerobic, rod-shaped and non-motile bacterium, PAMC 27130(T), was isolated from the marine sediment of the Ross Sea, Antarctica. The temperature, pH and NaCl tolerance ranges for growth were 4-20 °C, pH 6.0-9.0 and 0.5-5.0 % (w/v) NaCl, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PAMC 27130(T) belonged to the genus Psychroserpens and was closely related to Psychroserpens mesophilus, Psychroserpens damuponensis and Psychroserpens burtonensis with 97.2, 94.7 and 94.2 % sequence similarities, respectively. Genomic relatedness analyses based on average nucleotide identity and genome-to-genome distance showed that strain PAMC 27130(T) could be clearly distinguished from other species of the genus Psychroserpens . The genomic DNA G+C content was 32.7 mol%. The major fatty acids (>10 %) were C20 : 4ω6c (13.2 %), iso-C15 : 0 (12.3 %), iso-C15 : 1 G (11.7 %) and iso-C15 : 0 3-OH (10.0 %). The major respiratory isoprenoid quinone was menaquinone-6 (MK-6) and the polar lipids were phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid, an unidentified aminophospholipid and three unidentified lipids. On the basis of genotypic and phenotypic data collected in this study, it is proposed that strain PAMC 27130(T) represents a novel species of the genus Psychroserpens, for which the name Psychroserpens jangbogonensis sp. nov. is proposed. The type strain is PAMC 27130(T) ( = KCTC 42128(T) = JCM 30228(T)).
一株革兰氏阴性、黄色、需氧、杆状且不运动的细菌，PAMC 27130(T)，从南极洲罗斯海的海洋沉积物中分离得到。其生长的温度、pH 和 NaCl 耐受范围分别为 4-20°C、pH 6.0-9.0 和 0.5-5.0%（w/v）NaCl。基于 16S rRNA 基因序列的系统发育分析表明，菌株 PAMC 27130(T)属于 Psychroserpens 属，与 Psychroserpens mesophilus、Psychroserpens damuponensis 和 Psychroserpens burtonensis 的相似度分别为 97.2%、94.7%和 94.2%。基于平均核苷酸同一性和基因组到基因组距离的基因组相关性分析表明，菌株 PAMC 27130(T)可以与 Psychroserpens 属的其他种明显区分开来。基因组 DNA G+C 含量为 32.7mol%。主要脂肪酸（>10%）为 C20：4ω6c（13.2%）、iso-C15：0（12.3%）、iso-C15：1 G（11.7%）和 iso-C15：0 3-OH（10.0%）。主要呼吸异戊烯醌为menaquinone-6（MK-6），极性脂类为磷脂酰乙醇胺、两种未鉴定的胺脂类、一种未鉴定的磷脂、一种未鉴定的胺磷脂和三种未鉴定的脂类。基于本研究中收集的基因型和表型数据，建议菌株 PAMC 27130(T)代表 Psychroserpens 属的一个新种，命名为 Psychroserpens jangbogonensis sp. nov.。模式菌株为 PAMC 27130(T)（=KCTC 42128(T)=JCM 30228(T)）。
