Department of Medical Microbiology & Infectious Diseases, Erasmus Medical Center Rotterdam, 's Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands.
Acta Vet Scand. 2012 May 1;54(1):28. doi: 10.1186/1751-0147-54-28.
During routinely screening (50.000 milk samples on an annual basis) 14 MRSA ST398 strains were identified in the period of January 2008 to September 2008 in 14 different dairy herds located in the provinces Overijssel and Gelderland, The Netherlands. Molecular analysis was performed by Cfr9I PFGE, ST398-specific diagnostic PCR, spa typing, SCCmec typing and Panton-Valentine Leukocidin (PVL) gene PCR. The molecular analyses of 14 MRSA (one MRSA strain per herd) strains revealed that all strains belong to ST398 with 3 closely related spa types (t011, t108 and t889, all commonly found in pigs) and carry 2 different SCCmec types, IVa and V. All MRSA strains were resistant to two or more classes of antibiotics and also PVL negative. The majority of farms (n = 9, 64%) harboured combined livestock with both cows and pigs present. Our study contributes to the growing evidence that MRSA ST398 is transmitted among various animal species and can be considered as an etiological agent of mastitis in dairy cows.
在对（每年 50000 个牛奶样本进行例行筛查）期间，于 2008 年 1 月至 2008 年 9 月在荷兰上艾瑟尔省和格尔德兰省的 14 个不同的奶牛场发现了 14 株耐甲氧西林金黄色葡萄球菌（MRSA）ST398 菌株。分子分析采用 Cfr9I PFGE、ST398 特异性诊断 PCR、spa 分型、SCCmec 分型和 Panton-Valentine Leukocidin（PVL）基因 PCR 进行。对 14 株 MRSA（每群一株 MRSA 株）的分子分析表明，所有菌株均属于 ST398，具有 3 种密切相关的 spa 型（t011、t108 和 t889，均常见于猪），并携带 2 种不同的 SCCmec 型，IVa 和 V。所有 MRSA 菌株均对 2 种或多种类别的抗生素具有耐药性，且均为 PVL 阴性。大多数农场（n=9，64%）同时饲养奶牛和猪。我们的研究为越来越多的证据表明，MRSA ST398 在各种动物物种之间传播，并可被视为奶牛乳腺炎的病原体。
