Ha Kevin D, Clarke Benjamin A, Brown William J
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Biochim Biophys Acta. 2012 Aug;1821(8):1078-88. doi: 10.1016/j.bbalip.2012.04.004. Epub 2012 Apr 22.
The mammalian Golgi complex is a highly dynamic organelle consisting of stacks of flattened cisternae with associated coated vesicles and membrane tubules that contribute to cargo import and export, intra-cisternal trafficking, and overall Golgi architecture. At the morphological level, all of these structures are continuously remodeled to carry out these trafficking functions. Recent advances have shown that continual phospholipid remodeling by phospholipase A (PLA) and lysophospholipid acyltransferase (LPAT) enzymes, which deacylate and reacylate Golgi phospholipids, respectively, contributes to this morphological remodeling. Here we review the identification and characterization of four cytoplasmic PLA enzymes and one integral membrane LPAT that participate in the dynamic functional organization of the Golgi complex, and how some of these enzymes are integrated to determine the relative abundance of COPI vesicle and membrane tubule formation. This article is part of a Special Issue entitled Lipids and Vesicular Transport.
哺乳动物的高尔基体是一种高度动态的细胞器，由堆叠的扁平囊泡以及相关的被膜小泡和膜小管组成，这些结构有助于货物的进出、囊泡内运输以及高尔基体的整体结构。在形态学层面，所有这些结构都在不断重塑以执行这些运输功能。最近的研究进展表明，磷脂酶A（PLA）和溶血磷脂酰转移酶（LPAT）分别对高尔基体磷脂进行去酰化和再酰化的持续磷脂重塑作用，有助于这种形态重塑。在此，我们综述了四种细胞质PLA酶和一种整合膜LPAT的鉴定与特性，它们参与了高尔基体复合体的动态功能组织，以及其中一些酶如何相互作用以确定COPI小泡和膜小管形成的相对丰度。本文是名为“脂质与囊泡运输”的特刊的一部分。
