基于反应的双光子汞离子探针：具有双光学窗口的荧光成像。

Reaction-based two-photon probes for mercury ions: fluorescence imaging with dual optical windows.

机构信息

Department of Chemistry and the Center for Electro-Photo Behaviors in Advanced Molecular Systems, Department of Mechanical Engineering and Division of Integrative Biosciences and Biotechnology, POSTECH , San 31, Hyoja-dong, Pohang, 790-784, Republic of Korea.

出版信息

Org Lett. 2012 May 18;14(10):2598-601. doi: 10.1021/ol3009057. Epub 2012 May 7.

DOI:10.1021/ol3009057

PMID:22564078

Abstract

For fluorescent imaging of mercury ions in living species, two-photon probes with dual optical windows are in high demand but remain unexplored. Several dithioacetals were evaluated, and a probe was found, which, upon reaction with mercury species, yielded a two-photon dye; this conversion accompanies ratiometric emission changes with a 97-nm shift, enabling fluorescent imaging of both the probe and mercury ions in cells by one- and two-photon microscopy for the first time.

摘要

为了在活体物种中实现汞离子的荧光成像，双光窗的双光子探针的需求很高，但尚未得到探索。评估了几种二硫缩醛，发现了一种探针，该探针与汞物种反应后生成了一种双光子染料; 这种转化伴随着比率发射变化和 97nm 的位移，使得可以首次通过单光子和双光子显微镜对探针和细胞中的汞离子进行荧光成像。