Araújo L U, Reis P G, Barbosa L C O, Saúde-Guimarães D A, Grabe-Guimarães A, Mosqueira V C F, Carneiro C M, Silva-Barcellos N M
Departamento de Farmácia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Brasil.
Pharmazie. 2012 Apr;67(4):355-60.
The present work evaluates wound healing activity of leaves extracts of Symphytum officinale L. (comfrey) incorporated in three pharmaceutical formulations. Wound healing activity of comfrey was determined by qualitative and quantitative histological analysis of open wound in rat model, using allantoin as positive control. Three topical formulations, carbomer gel, glycero-alcoholic solution and O/W emulsion (soft lotion) were compared. The histological analysis of the healing process shows significant differences in treatment, particularly on its intensity and rate. The results indicate that emulsion containing both extracts, commercial and prepared, induced the largest and furthest repair of damaged tissue. This could be evidenced from day 3 to 28 by increase in collagen deposition from 40% to 240% and reduction on cellular inflammatory infiltrate from 3% to 46%. However, 8% prepared extract in emulsion presented the best efficacy. This work clearly demonstrates that comfrey leaves have a wound healing activity. The O/W emulsion showed to be the vehicle most effective to induce healing activity, particularly with extracts obtained from comfrey leaves collected in Minas Gerais state in Brazil. It shows the best efficacy to control the inflammatory process and to induce collagen deposition at 8% concentration.
本研究评估了三种药物制剂中掺入的药用紫草（聚合草）叶提取物的伤口愈合活性。以尿囊素作为阳性对照，通过对大鼠模型开放性伤口进行定性和定量组织学分析，确定了聚合草的伤口愈合活性。比较了三种外用制剂，即卡波姆凝胶、甘油醇溶液和水包油乳液（软洗剂）。愈合过程的组织学分析显示，治疗方法存在显著差异，尤其是在治疗强度和速率方面。结果表明，含有市售提取物和自制提取物的乳液诱导受损组织的修复范围最大、程度最深。从第3天到第28天，这一点可通过胶原蛋白沉积从40%增加到240%以及细胞炎性浸润从3%减少到46%得到证明。然而，乳液中8%的自制提取物表现出最佳疗效。这项研究清楚地表明，聚合草叶具有伤口愈合活性。水包油乳液被证明是诱导愈合活性最有效的载体，尤其是对于从巴西米纳斯吉拉斯州采集的聚合草叶中获得的提取物。它在8%浓度下显示出控制炎症过程和诱导胶原蛋白沉积的最佳疗效。
