Schranz V, Kulcsár G, Dán P, Horváth J, Nász I, Barinsky I F, Ugryumov E P
Acta Microbiol Acad Sci Hung. 1979;26(1):1-9.
Interaction between phytohaemagglutinin (PHA) stimulated human lymphocytes and two DNA viruses (adenovirus type 5 and herpes simplex virus type 1) considered to be closely connected with lymphoid tissues has been studied. The fate of the same viruses was investigated also in non-stimulated separated lymphocytes for comparative purposes. To elicit this interaction infectivity titrations, immunofluorescent technique and electron microscopy were used. The production of viral antigens was investigated by complement fixation. It has been shown that in PHA-stimulated lymphocytes from peripheral blood of healthy donors adenovirus type 5 is capable to replicate in its infectious form. Prolongation of the interval between stimulation and infection of cells significantly influenced the dynamics of replication. Non-stimulated lymphocytes produced antigens but no infectious particles.
对植物血凝素（PHA）刺激的人淋巴细胞与两种被认为与淋巴组织密切相关的DNA病毒（5型腺病毒和1型单纯疱疹病毒）之间的相互作用进行了研究。为作比较，还对未受刺激的分离淋巴细胞中这两种病毒的情况进行了研究。为引发这种相互作用，采用了感染性滴定、免疫荧光技术和电子显微镜检查。通过补体结合试验研究病毒抗原的产生。结果表明，在健康供体外周血经PHA刺激的淋巴细胞中，5型腺病毒能够以感染性形式进行复制。延长细胞刺激与感染之间的间隔时间会显著影响复制动态。未受刺激的淋巴细胞产生抗原，但不产生感染性颗粒。
